Human SNAP25 antibody

The synaptosomal-associated protein (SNAP25) is an essential component of the core complex that mediates presynaptic vesicle trafficking. Thus, SNAP25 is directly involved in the release of neurotransmitters.

Product Specifications

Product Name Alternative

Synaptosomal-associated protein 25 isoform SNAP25B, SNAP25, SNAP, SNAP25, Synaptosomal-associated protein, His- tag, SNAP-25, Synaptosomal-associated 25 kDa protein, Super protein, SuP, Synaptosomal-associated protein 25, Synaptosomal-associated protein 25 isoform SNAP25B bA416N4.2, Bdr, dJ1068F16.2, FLJ23079, HGNC:11132, MGC105414, MGC139754, Resistance to inhibitors of cholinesterase 4 homolog, RIC 4, RIC4, SEC9, SNAP 25, SNAP-25B, SNP 25, sp, Synaptosomal associated 25 kDa protein, Synaptosomal associated protein, Synaptosomal Associated Protein 25, Synaptosomal associated protein 25kDa

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human SNAP25 (1-206aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

400000000000

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

SNAP25, ASN0720-10 µg, ASN0720-20 µg, ASN0720-50 µg, ASN0720-100 µg, ASN0720-250 µg, ASN0720-500 µg, ASN0720-1 mg, ASN0720-10, ASN0720-20, ASN0720-50, ASN0720-100, ASN0720-250, ASN0720-500, ASN0720-1

References & Citations

Schulz JR et al., (1998) J Biol Chem 273 (38) :24355-9.; ; Tafoya LC et al., (2006) J Neurosci 26 (30) :7826-38.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Neuroscience

NCBI Accession Number

NP_570824

Uniprot Accession Number

P60880

WB Description

The tissue lysate (30ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SNAP25 antibody (1:2000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Mouse brain tissue lysate The cell lysates (10 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SNAP25 antibody (1:3000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: SNAP25 Transfected 293T cell lysate {2004010049531236.jpg}

IF Description

ICC/IF analysis of SNAP25 in U87MG cells. The cell was stained with ASN0720 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .