Human RASSF1A antibody

RASSF1A, ARF0501-10 µg, ARF0501-20 µg, ARF0501-50 µg, ARF0501-100 µg, ARF0501-250 µg, ARF0501-500 µg, ARF0501-1 mg, ARF0501-10, ARF0501-20, ARF0501-50, ARF0501-100, ARF0501-250, ARF0501-500, ARF0501-1

Agathanggelou A, et al., (2005) Cancer Res. 65, 3497-3508.; ; Lerman MI. et. al. (2000) Cancer Res. 60, 6116-6133.; ; Strunnikova M. et al., (2005) Mol Cell Biol. 25, 3923-3933.; ; Dammann R. et al., (2005) Histol Histopathol. 20, 645-663.; ; Rossa W.k, et al., (2007) American Journal of pathology. 170, 941-950.

Thaler S, et al. RASSF1A inhibits estrogen receptor alpha expression and estrogen-independent signalling: implications for breast cancer development. (Oncogene. 2012) {https://pubmed.ncbi.nlm.nih.gov/22266866/}; ; Dratviman-Storobinsky O, et al. The role of RASSF1A in uveal melanoma. (Invest Ophthalmol Vis Sci. 2012) {https://pubmed.ncbi.nlm.nih.gov/22447862/}; ; Gou HF, et al. Epidermal growth factor receptor (EGFR) -RAS signaling pathway in penile squamous cell carcinoma. (PLoS One. 2013) {https://pubmed.ncbi.nlm.nih.gov/23637996/}; ; Korah R, et al. Epigenetic silencing of RASSF1A deregulates cytoskeleton and promotes malignant behavior of adrenocortical carcinoma. (Mol Cancer. 2013) {https://pubmed.ncbi.nlm.nih.gov/23915220/}; ; Beckedorff FC, et al. The intronic long noncoding RNA ANRASSF1 recruits PRC2 to the RASSF1A promoter, reducing the expression of RASSF1A and increasing cell proliferation. (PLoS Genet. 2013) {https://pubmed.ncbi.nlm.nih.gov/23990798/}; ; Ram RR, et al. RASSF1A inactivation unleashes a tumor suppressor/oncogene cascade with context-dependent consequences on cell cycle progression. (Mol Cell Biol. 2014) {https://pubmed.ncbi.nlm.nih.gov/24732797/}; ; Pruikkonen S, Kallio MJ. Excess of a Rassf1-targeting microRNA, miR-193a-3p, perturbs cell division fidelity. (Br J Cancer. 2017) {https://pubmed.ncbi.nlm.nih.gov/28449010/}; ; Li W, et al. Suppressor of hepatocellular carcinoma RASSF1A activates autophagy initiation and maturation. (Cell Death Differ. 2019) {https://pubmed.ncbi.nlm.nih.gov/30315205/}; ; Guo M, et al. RASSF1-AS1, an antisense lncRNA of RASSF1A, inhibits the translation of RASSF1A to exacerbate cardiac fibrosis in mice. (Cell Biol Int. 2019) {https://pubmed.ncbi.nlm.nih.gov/30571844/}; ; Dabral S, et al. A RASSF1A-HIF1α loop drives Warburg effect in cancer and pulmonary hypertension. (Nat Commun. 2019) {https://pubmed.ncbi.nlm.nih.gov/31086178/}; ; Francisco J, et al. The tumor suppressor RASSF1A modulates inflammation and injury in the reperfused murine myocardium. (J Biol Chem. 2019) {https://pubmed.ncbi.nlm.nih.gov/31311858/}; ; Chen J, et al. 5-Aza-CdR Regulates RASSF1A By Inhibiting DNMT1 To Affect Colon Cancer Cell Proliferation, Migration And Apoptosis. (Cancer Manag Res. 2019) {https://pubmed.ncbi.nlm.nih.gov/31807076/}; ; Slemmons KK, et al. Targeting Hippo-Dependent and Hippo-Independent YAP1 Signaling for the Treatment of Childhood Rhabdomyosarcoma. (Cancer Res. 2020) {https://pubmed.ncbi.nlm.nih.gov/32354737/}; ; Dhanaraman T, et al. RASSF effectors couple diverse RAS subfamily GTPases to the Hippo pathway. (Sci Signal. 2020) {https://pubmed.ncbi.nlm.nih.gov/33051258/}; ; Jeon HJ, Oh JS. RASSF1A Regulates Spindle Organization by Modulating Tubulin Acetylation via SIRT2 and HDAC6 in Mouse Oocytes. (Front Cell Dev Biol. 2020) {https://pubmed.ncbi.nlm.nih.gov/33195286/}; ; Jia J, et al. RAS-association domain family 1A regulates the abnormal cell proliferation in psoriasis via inhibition of Yes-associated protein. (J Cell Mol Med. 2021) {https://pubmed.ncbi.nlm.nih.gov/33960627/}; ; Roßwag S, et al. RASSF1A-Mediated Suppression of Estrogen Receptor Alpha (ERα) -Driven Breast Cancer Cell Growth Depends on the Hippo-Kinases LATS1 and 2. (Cells. 2021) {https://pubmed.ncbi.nlm.nih.gov/34831091/}

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RASSF1A (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysateCell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RASSF1A (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: RASSF1A transfected 293T cell lysate{2004010525238693.jpg}

ICC/IF analysis of RASSF1A in HeLa cells. The cell was stained with ARF0501 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of RASSF1A in A431 cells. The cell was stained with ARF0501 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{ARF0501-IF2.jpg}