Human RAB5A antibody

Rab5a is a member of the RAS oncogene family. Rab5a is a small GTPase localized to the plasma membrane and early endosomes. It is a crucial regulatory component of the endocytic pathway. Activation of Rab5a is mediated by GDP-GTP exchange factors (GEFs) that generate the Rab5-GTP complex.

Product Specifications

Product Name Alternative

RAB5A member RAS oncogene family, Ras-related protein Rab-5A, RAS-associated protein RAB5A, RAB5

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human Rab5a (1-215aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

3A4

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Rab5a, ARA0803-10 µg, ARA0803-20 µg, ARA0803-50 µg, ARA0803-100 µg, ARA0803-250 µg, ARA0803-500 µg, ARA0803-1 mg, ARA0803-10, ARA0803-20, ARA0803-50, ARA0803-100, ARA0803-250, ARA0803-500, ARA0803-1

References & Citations

Bucci C, et al., (1992) Cell 70 (5) : 715-28.; ; Seachrist JL, et al., (2002) J. Biol. Chem. 277 (1) : 679-85.

Other References

Del Cid-Pellitero E, et al. Homeostatic Changes in GABA and Glutamate Receptors on Excitatory Cortical Neurons during Sleep Deprivation and Recovery. (Front Syst Neurosci. 2017) {https://pubmed.ncbi.nlm.nih.gov/28408870/}; ; Winer H, et al. Autophagy differentially regulates TNF receptor Fn14 by distinct mammalian Atg8 proteins. (Nat Commun. 2018) {https://pubmed.ncbi.nlm.nih.gov/30218067/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cancer

NCBI Accession Number

NP_004153

Uniprot Accession Number

P20339

WB Description

The tissue lysate of mouse brain (45ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Rab5a (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

IF Description

ICC/IF analysis of Rab5a in U87MG cells. The cell was stained with ARA0803 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .