Human Folate hydrolase 1/FOLH1 antibody

Prostate-specific membrane antigen (PSMA) is a 100-120 kDa type II transmembrane glycoprotein that is a glutamate-preferring carboxy-peptidase. Three functionally distinct proteins are encoded, including folylpoly-gamma-glutamate carboxypeptidase in the intestine, N-acetylated alpha-linked acidic dipeptidase 1 in the brain, and prostate-specific membrane antigen in the prostate. PSMA is highly expressed in prostate secretory-acinar epithelium, in some benign extraprostatic epithelial cells from breast, duodenum, and kidney tissues, and in prostate cancer.

Product Specifications

Product Name Alternative

Glutamate carboxypeptidase 2, Cell growth-inhibiting gene 27 protein, Folylpoly-gamma-glutamate carboxypeptidase, FGCP, Glutamate carboxypeptidase II, GCPII, Membrane glutamate carboxypeptidase, mGCP, N-acetylated-alpha-linked acidic dipeptidase I, NAALADase I, Prostate-specific membrane antigen, PSM, PSMA, Pteroylpoly-gamma-glutamate carboxypeptidase, FOLH, Naalad1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human PSMA (117-351aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

K1H7

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

PSMA, FOLH1, NAALADase I, APS0819-10 µg, APS0819-20 µg, APS0819-50 µg, APS0819-100 µg, APS0819-250 µg, APS0819-500 µg, APS0819-1 mg, APS0819-10, APS0819-20, APS0819-50, APS0819-100, APS0819-250, APS0819-500, APS0819-1

References & Citations

Rajasekaran SA, et al., (2008) Mol Cancer Ther 7 (7) :2142-2151.; ; Polly D.G, et al., (2005) Int J Cancer 116 (3) :415-421.; ; Susan F.S, et al., (2005) Expert Opin Ther Targets 9 (3) :561-570.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cancer

NCBI Accession Number

NP_001014986

Uniprot Accession Number

Q04609

WB Description

The Cell lysate of LNCaP (30ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PSMA (1:1,000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

IF Description

ICC/IF analysis of PSMA in LNCaP cells. The cell was stained with APS0819 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .