Human PP2C gamma/PPM1G antibody

PPM1G (Protein phosphatase 1G) is a member of the PP2C family of Serine/threonine protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The human Ser/Thr phosphatase PPM1G promotes spliceosome assembly. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome.

Product Specifications

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human PPM1G (317-546aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

K1G6

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, IHC

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Protein phosphatase, Mg2+/Mn2+ dependent 1G, Protein phosphatase Mg2+/Mn2+ dependent 1G, Protein phosphatase magnesium-dependent 1 gamma, Protein phosphatase magnesium dependent 1 gamma, Protein phosphatase 2C gamma isoform, Protein phosphatase 2 catalytic subunit gamma isoform, Protein phosphatase 1G, formerly 2C, magnesium-dependent, gamma isoform, Protein phosphatase 1G, Protein phosphatase 1C, PPP2CG, PPM1G, PPM1C, PP2Cgamma, PP2CG, PP2C gamma, PP2C g, PP2C, MGC2870, MGC1675, APP0902-10 µg, APP0902-20 µg, APP0902-50 µg, APP0902-100 µg, APP0902-250 µg, APP0902-500 µg, APP0902-1 mg, APP0902-010, APP0902-020, APP0902-050, APP0902-100, APP0902-250, APP0902-500, APP0902-01M

References & Citations

Suh EJ., et al. (2006) FEBS Lett 580 (26) :6100-6104.; ; Marc Flajolet., et al. (2003) Proc Natl Acad Sci 100 (26) :16006-16011.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_002698

Uniprot Accession Number

O15355

WB Description

The 293T cell lysate (20 µg) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPM1G antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

IHC description

Paraffin embedded sections of human breast cancer tissue were incubated with anti-human PPM1G (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

IF Description

ICC/IF analysis of PPM1G in 293T cells. The cell was stained with APP0902 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .