Human CPI-17/PPP1R14A antibody

PPP1R14A (Protein phosphatase 1 regulatory subunit 14A) is a phosphorylation-dependent inhibitory protein for smooth muscle myosin phosphate. Myosin phosphatase can reverse MYL (myosin light chain) phosphorylation to induce a state of relaxation. However, during agonist-induced contraction at constant Ca2+ concurrent inhibition of myosin phosphatase leads to increases in MYL phosphorylation and tension. These calcium-independent increases in myosin phosphorylation and tension are termed calcium sensitization.

Product Specifications

Product Name Alternative

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human PPP1R14A (1-147aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

4H10

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Regulatory subunit 14A, Regulatory (inhibitor) subunit 14A CPI 17, Regulatory (inhibitor) subunit 14A, Protein phosphatase 1 regulatory subunit 14A, Protein phosphatase 1 regulatory (inhibitor) subunit 14A, Protein phosphatase 1, PPP1R14A, PPP1INL, PKC potentiated inhibitory protein of PP1, CPI-17, CPI17, CPI 17alpha, CPI 17 alpha, CPI-17 alpha, CPI17 alpha, APP0832-10 µg, APP0832-20 µg, APP0832-50 µg, APP0832-100 µg, APP0832-250 µg, APP0832-500 µg, APP0832-1 mg, APP0832-010, APP0832-020, APP0832-050, APP0832-100, APP0832-250, APP0832-500, APP0832-01M

References & Citations

Hamaguchi., et al. (2000) Biochem. Biophys. Res Commun. 274 (3) :825-30.; ; Eto M., et al. (1997) FEBS Lett. 410 (2-3) :356-60.; ; Lartey I., et al. (2007) Biol Reprod. 76 (6) :971-82.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_150281

Uniprot Accession Number

Q96A00

WB Description

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPP1R14A antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : A549 cell lysate ; ; Lane 2. : NIH-3T3 cell lysate ;

IF Description

ICC/IF analysis of PPP1R14A in A549 cells. The cell was stained with APP0832 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .