Human PP2C alpha/PPM1A antibody

Protein phosphatase 2C (PP2Calpha) is a Mn2+ - or Mg 2+ -dependent protein serine/threonine phosphatase that inhibits the human stress-responsive p38 and JNk MAPk pathways and regulates cellular stress response in eukaryotes. The PPM (metal-dependent protein phosphatase) family of Ser/Thr protein phosphatases have recently been shown to down-regulate the stress response pathways in eukaryotes. Within the stress pathway, key signaling kinases, which are activated by protein phosphorylation, have been proposed as the in vivo substrates of PP2Calpha, the prototypical member of the PPM family.

Product Specifications

Product Name Alternative

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human PP2Calpha (1-382aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

P6c7

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Pyruvate dehydrogenase phosphatase catalytic subunit 1, Pyruvate dehydrogenase acetyl-transferring-phosphatase 1, Protein phosphatase Mg2+/Mn2+dependent 1A, Protein phosphatase 2C, Protein phosphatase 1A (formerly 2C) magnesium-dependent alpha isoform, Protein phosphatase 1A (formerly 2C), PPM2C, PPM1A, PP2C-alpha, PP2Calpha, PP2CA, PP2C A, PP2C alpha, PDPC 1, PDP1, PDP 1, PDP, MGC9201, FLJ42306, EC 3.1.3.43, APP0401-10 µg, APP0401-20 µg, APP0401-50 µg, APP0401-100 µg, APP0401-250 µg, APP0401-500 µg, APP0401-1 mg, APP0401-010, APP0401-020, APP0401-050, APP0401-100, APP0401-250, APP0401-500, APP0401-01M

References & Citations

Lin X, et al., (2006) Cell. 125 (5) : 915-928; ; Duan X, et al., (2006) J Biol Chem. 281 (48) :36526-32

Other References

Kim JS, et al. The interaction of hepatitis B virus X protein and protein phosphatase type 2 Calpha and its effect on IL-6. (Biochem Biophys Res Commun. 2006) {https://pubmed.ncbi.nlm.nih.gov/17055456/}; ; Kim YG, et al. Role of protein phosphatase magnesium-dependent 1A and anti-protein phosphatase magnesium-dependent 1A autoantibodies in ankylosing spondylitis. (Arthritis Rheumatol. 2014) {https://pubmed.ncbi.nlm.nih.gov/24980965/}; ; Pereira JM, et al. Infection Reveals a Modification of SIRT2 Critical for Chromatin Association. (Cell Rep. 2018) {https://pubmed.ncbi.nlm.nih.gov/29694890/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtainoptimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_066283

Uniprot Accession Number

P35813

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PP2C alpha antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Jurkat cell lysate; ; Lane 2.: HeLa cell lysate; ; Lane 3.: K562 cell lysate; ; Lane 4.: MCF7 cell lysate; ; Lane 5.: A549 cell lysate; ; Lane 6.: Raji cell lysate The tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PP2C alpha antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Mouse kidney tissue lysate; ; Lane 2.: Mouse brain tissue lysate; ; Lane 3.: Mouse liver tissue lysate {APP0401-WB2.jpg}

IF Description

ICC/IF analysis of PP2C alpha in HeLa cells. The cell was stained with APP0401 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .