Human Pin1 antibody

Human Pin 1 is a peptidyl-prolyl cis/trans isomerase (PPIase) that interacts with NIMA and essential for cell cycle regulation. Pin1 is nuclear PPIase containing a WW protein interaction domain, and is structurally and functionally related to Ess1/Ptf1, an essential protein in budding yeast. PPIase activity is necessary for Ess1/Pin1 function in yeast. Pin1 is thus an essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Substrates of Pin1 include the mitotic regulators (Cdc25 phosphatase and NIMA, PLk I, Wee, and Myt1 kinases), several transcription factors likebeta-Catenin, c-Jun, and the tumor suppressor protein p53, and some specific proteins like the RNA Pol II, the cytoskeleton protein tau, and the G1/S protein Cyclin D1.

Product Specifications

Product Name Alternative

Protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1, PIN1, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, EC 5.2.1.8, Rotamase Pin1, PPIase Pin1, DOD, uBL5, PIN1, PPIase, EC 5.2.1.8, Rotamase Pin1, PPIase Pin1, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human Pin1 (1-163aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

3G8

Conjugation

Unconjugated

Applications

ELISA, WB, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Pin1, API0622-10 µg, API0622-20 µg, API0622-50 µg, API0622-100 µg, API0622-250 µg, API0622-500 µg, API0622-1 mg, API0622-10, API0622-20, API0622-50, API0622-100, API0622-250, API0622-500, API0622-1

References & Citations

Wulf GM, et al., (2002) J Biol. Chem. 277 (50) :47976-47979.; ; Hamdane M, et al., (2002) J Mol Neurosci. 19 (3) :275-287.; ; Zheng H, et al., (2002) Nature 419 (6909) :853-857.; ; Lu KP. et al., (1996) Nature 380 (6574) :544-547.; ; Campbell HD, et al., (1997) Genomics 44 (2) :157-162

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Neuroscience

NCBI Accession Number

NP_006212

Uniprot Accession Number

Q13526

WB Description

The cell lysates (50 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Pin1 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: HeLa cell lysate; ; Lane 3: Jurkat cell lysate;

FACS Description

Flow cytometry analysis of Pin1 in Jurkat cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .