Human PDCD12 antibody

PDCD12 (Programmed cell death 12), also referred to as AVEN, represents a new class of cell death regulator. PDCD12 is a conserved intracellular membrane protein that blocks apoptosis impeded by Apaf-1 mediated caspase activation. Bcl-2 and BAD also interact with PDCD12 to prevent apoptosis. It is expressed in a wide variety of normal tissues including heart, skeletal muscle, kidney, liver, testis, and diseases such as cancer

Product Specifications

Product Name Alternative

Cell death regulator aven, apoptosis, caspase activation inhibitor, PDCD12, programmed cell death 12

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human PDCD12 (254-362aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

3G4

Conjugation

Unconjugated

Applications

ELISA, WB, IHC

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

PDCD12, APD0912-10 µg, APD0912-20 µg, APD0912-50 µg, APD0912-100 µg, APD0912-250 µg, APD0912-500 µg, APD0912-1 mg, APD0912-10, APD0912-20, APD0912-50, APD0912-100, APD0912-250, APD0912-500, APD0912-1

References & Citations

Figueroa B Jr, et al: (2004) Biotechnol Bioeng. 85 (6), 589-600.; ; Cheng EH, et al: (2000) Mol Cell. 6 (1), 31-40.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cancer

NCBI Accession Number

NP_065104

Uniprot Accession Number

Q9NQS1

WB Description

The tissue lysate of mouse kidney (50 µg) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PDCD12 (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

IHC description

Paraffin embedded sections of human breast lobule tissue were incubated with anti-human PDCD12 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)