Human PCNA antibody

PCNA (Proliferating Cell Nuclear Antigen) is found in the nucleus and is a cofactor of DNA polymerase delta. This protein is associated with DNA synthesis and repair. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. It appears during late G1- phase, S-phase of mitosis and persists until the end of the M-phase because of its long biological half-life. PCNA may be induced by UV irradiation, growth factors and eventually by neighboring tumors.

Product Specifications

Product Name Alternative

Proliferating cell nuclear antigen, MGC8367, Proliferating cell nuclear antigen, Proliferating cell nuclear antigen, PCNA

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human PCNA (1-261aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG3 κ

Clone

K1A3

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

PCNA, APC0828-10 µg, APC0828-20 µg, APC0828-50 µg, APC0828-100 µg, APC0828-250 µg, APC0828-500 µg, APC0828-1 mg, APC0828-10, APC0828-20, APC0828-50, APC0828-100, APC0828-250, APC0828-500, APC0828-1

References & Citations

Moldovan GL, et al., (2007) Cell 18 129 (4) :665-679.; ; Matsumoto K , et al., (1987) EMBO 6 (3) :637-642.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cell Cycle

NCBI Accession Number

NP_002583

Uniprot Accession Number

P12004

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PCNA (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: NIH3T3 cell lysate