Human HTRA2/OMI (S306A) antibody

HtrA2/Omi is a mammalian serine protease at high temperatures and a chaperone at low temperature. The full-length HtrA2 is synthesized as a precursor protein and then targeted to the mitochondria where it is matured by the removal of N-terminal 133 residues. Mature HtrA2 consists of a putative transmembrane domain; an inhibitor of apoptosis protein (IAP) -binding motif; a single C-terminal PDZ domain that mediates protein-protein interactions. Recently, HtrA2 has known to contribute both to caspase-dependent and caspase-independent cell death.

Product Specifications

Product Name Alternative

HtrA serine peptidase 2, High temperature requirement protein A2, HtrA2, Omi stress-regulated endoprotease, Serine protease 25, Serine proteinase OMI, PRSS25, OMI, PARK13

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human HtrA2/Omi (134-458aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

1B3

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

HTRA2, Omi, AOH0607-10 µg, AOH0607-20 µg, AOH0607-50 µg, AOH0607-100 µg, AOH0607-250 µg, AOH0607-500 µg, AOH0607-1 mg, AOH0607-10, AOH0607-20, AOH0607-50, AOH0607-100, AOH0607-250, AOH0607-500, AOH0607-1

References & Citations

Miller L., et al., (1999) Trends Cell Biol. 9:323-28.; ; Zumbrunn J., et al., (1996) FABS Lett. 398:187-92.; ; Gui-Ying N., et al., (2003) Biochem. J. 371:39-48.

Other References

Kim GY, et al. A simple and rapid strategy for the molecular cloning and monitoring of mouse HtrA2 serine protease. (Biotechnol Lett. 2008) {https://pubmed.ncbi.nlm.nih.gov/17939055/}; ; Park HJ, et al. Intracellular Abeta and C99 aggregates induce mitochondria-dependent cell death in human neuroglioma H4 cells through recruitment of the 20S proteasome subunits. (Brain Res. 2009) {https://pubmed.ncbi.nlm.nih.gov/19362074/}; ; Shin EJ, et al. Matrix metalloproteinase-3 is activated by HtrA2/Omi in dopaminergic cells: relevance to Parkinsons disease. (Neurochem Int. 2012) {https://pubmed.ncbi.nlm.nih.gov/22265821/}; ; Goo HG, et al. HtrA2/Omi deficiency causes damage and mutation of mitochondrial DNA. (Biochim Biophys Acta. 2013) {https://pubmed.ncbi.nlm.nih.gov/23542127/}; ; Goo HG, et al. HtrA2/Omi influences the stability of LON protease 1 and prohibitin, proteins involved in mitochondrial homeostasis. (Exp Cell Res. 2014) {https://pubmed.ncbi.nlm.nih.gov/25094062/}; ; Nam MK, et al. HtrA2 regulates α-Synuclein-mediated mitochondrial reactive oxygen species production in the mitochondria of microglia. (Biochem Biophys Res Commun. 2023) {https://pubmed.ncbi.nlm.nih.gov/36442236/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cancer

NCBI Accession Number

NP_037379

Uniprot Accession Number

O43464

WB Description

The cell lysates of 293T, MCF7, HT-29, HL-60, HeLa and tissue lysates of mouse brain, rat brain were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HtrA2/Omi antibody (1:1,000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.