Human NSD3 antibody

This gene encoding NSD3 is related to the Wolf-Hirschhorn syndrome candidate - 1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. NSD3 is Histone methyltransferase which preferentially methylates 'Lys-4' and 'Lys-27' of histone H3 and is amplified in several tumor cell lines and primary breast carcinomas.

Product Specifications

Product Name Alternative

Transcription elongation factor B polypeptide 3 binding protein 1, DKFZp667H044, FLJ20353, Histone lysine N methyltransferase NSD3, MGC126766, MGC142029, NSD 3, Nuclear set domain containing 3, Nuclear SET domain containing protein 3, pp14328, Whistle, WHSC1 like protein 1, WHSC1L1 protein, Wolf Hirschhorn syndrome candidate 1 like 1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human NSD3 (383-660aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT7B4

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

NSD3, Transcription elongation factor B polypeptide 3 binding protein 1, DKFZp667H044, FLJ20353, Histone lysine N methyltransferase NSD3, MGC126766, MGC142029, NSD 3, Nuclear set domain containing 3, Nuclear SET domain containing protein 3, pp14328, Whistle, WHSC1 like protein 1, WHSC1L1 protein, Wolf Hirschhorn syndrome candidate 1 like 1, ANS0804-10 µg, ANS0804-20 µg, ANS0804-50 µg, ANS0804-100 µg, ANS0804-250 µg, ANS0804-500 µg, ANS0804-1 mg, ANS0804-10, ANS0804-20, ANS0804-50, ANS0804-100, ANS0804-250, ANS0804-500, ANS0804-1

References & Citations

Angrand P.O, et al. (2001) Genomics 74 (1) :79-88; ; Stec I, et al. (2000) FEBS Lett 473 (1) :1-5

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_075447

Uniprot Accession Number

Q9BZ95

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NSD3 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate ; ; Lane 2.: HeLa cell lysate ; ; Lane 3.: MCF7 cell lysate ; ; Lane 4.: NIH3T3 cell lysate ; ; Lane 5.: U87MG cell lysate