Human NPM1 antibody

Nucleophosmin (NPM; also known as B23, NO38, or numatrin) is a nucleolar phosphoprotein that binds the tumor suppressors p53 and p19Arf and is thought to be indispensable for ribogenesis, cell proliferation, and survival after DNA damage. Nucleophosmin is upregulated in many cancer cells.

Product Specifications

Product Name Alternative

Nucleophosmin (nucleolar phosphoprotein B23, numatrin), B23, NPM, MGC104254, nucleolar phosphoprotein B23, numatrin, nucleophosmin/nucleoplasmin family, member 1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human NPM (81-294aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

5000

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, IHC, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

NPM1, ANP0626-10 µg, ANP0626-20 µg, ANP0626-50 µg, ANP0626-100 µg, ANP0626-250 µg, ANP0626-500 µg, ANP0626-1 mg, ANP0626-10, ANP0626-20, ANP0626-50, ANP0626-100, ANP0626-250, ANP0626-500, ANP0626-1

References & Citations

Maiguel DA et al., (2004) Mol cell Biol 24:3703-3711.; ; Colombo Emanuela et al., (2005) Mol cell Biol 25:8874-8886.

Other References

Huang M, et al. Autophagy mediates proteolysis of NPM1 and HEXIM1 and sensitivity to BET inhibition in AML cells. (Oncotarget. 2016) {https://pubmed.ncbi.nlm.nih.gov/27732946/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_002511

Uniprot Accession Number

P06748

WB Description

The HeLa and HepG2 cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NPM (1:1,000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

IHC description

This image shows paraffin-embedded human palatine tonsil tissue sample stained with anti-NPM antibody (5E3) at 1:100 dilution.

FACS Description

Flow cytometry analysis of NPM in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of NPM in HeLa cells. The cell was stained with ANP0626 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .