Human Nanog antibody

Nanog is a multidomain homeobox transcription factor that functions to maintain the undifferentiated state of pluripotent stem cells. Nanog expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP. When Nanog expression is downregulated, cell differentiation can proceed. Proteins that regulate Nanog expression include transcription factors Oct4, SOX2, FoxD3, and Tcf3 and tumor suppressor p53.

Product Specifications

Product Name Alternative

Nanog homeobox, hNanog, Homeobox protein NANOG, Homeobox transcription factor Nanog

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human Nanog (1-154aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

5A10

Conjugation

Unconjugated

Applications

ELISA, WB, IHC, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Nanog homeobox, Nanog, Homeobox transcription factor Nanog-delta 48, Homeobox transcription factor Nanog, Homeobox protein NANOG, hNanog, FLJ40451, FLJ12581, , , ANA0837-10 µg, ANA0837-20 µg, ANA0837-50 µg, ANA0837-100 µg, ANA0837-250 µg, ANA0837-500 µg, ANA0837-1 mg, ANA0837-010, ANA0837-020, ANA0837-050, ANA0837-100, ANA0837-250, ANA0837-500, ANA0837-01M

References & Citations

Tay Y., et al. (2008) Nature. 455 (7216) :1124-8; ; Chiou SH., et al. (2008) Clin Cancer Res. 14 (13) :4085-95; ; Boer B., et al. (2008) Mol Reprod Dev.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Immunology

NCBI Accession Number

NP_079141

Uniprot Accession Number

Q9H9S0

WB Description

The cell lysate of NIH3T3 (35ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Nanog (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

IHC description

Paraffin embedded sections of human seminoma tissue were incubated with anti-human Nanog (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

FACS Description

Flow cytometry analysis of Nanog in Hep3B cell line, staining at 2-5ug for 1x10^6cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.