Human MIF antibody

The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration.

Product Specifications

Product Name Alternative

Macrophage migration inhibitory factor, GLIF, MMIF, MIF, EC 5.3.2.1, Phenylpyruvate tautomerase, Glycosylation-inhibiting factor, GIF, Macrophage migration inhibitory factor, macrophage migration inhibitory factor (glycosylation-inhibiting factor)

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human MIF (1-115 aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

40000

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

MIF, AMF0608-10 µg, AMF0608-20 µg, AMF0608-50 µg, AMF0608-100 µg, AMF0608-250 µg, AMF0608-500 µg, AMF0608-1 mg, AMF0608-10, AMF0608-20, AMF0608-50, AMF0608-100, AMF0608-250, AMF0608-500, AMF0608-1

References & Citations

Weiser WY, et al., (1989) Proc Natl Acad Sci. 86:7522-7526.; ; Bernhagen J, et al., (1994) Biochemistry. 33:14144-14155.; ; Richard B, et al., (1996) FASEB J. 10:1607-1613.

Other References

Koizumi M, et al. Positive correlations of age and parity with plasma concentration of macrophage migration inhibitory factor in Japanese black cows. (J Reprod Dev. 2016) {https://pubmed.ncbi.nlm.nih.gov/26853787/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Immunology

NCBI Accession Number

NP_002406

Uniprot Accession Number

P14174

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MIF antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Jurkat cell lysate; ; Lane 2.: THP-1 cell lysate; ; Lane 3.: HeLa cell lysate; ; Lane 4.: U937 cell lysate The cell lysates of HL-60 was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MIF antibody (1:1,000) . Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. {AMF0608-WB2.jpg}

IF Description

ICC/IF analysis of MIF in Balb/3T3 cells. The cell was stained with AMF0608 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of MIF in HeLa cells. The cell was stained with AMF0608 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{AMF0608-IF2.jpg}