E.coli Maltose Binding Protein/MBP antibody

Epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product.

Product Specifications

Product Name Alternative

ZCSL3, MMBP, MBP, Maltose binding protein, Maltodextrin-binding protein, malJ, malE, JW3994, JJJ3, Escherichia coli MBP, ECK4026, E.coli MBP, DPH4, Cytoplasmic maltose-binding protein

Host

Mouse

Antigen Species

E.coli

Immunogen

Recombinant MBP purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

10C12

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Maltose binding protein, malE, E.coli MBP, Escherichia coli MBP, MBP, AMB0710-10 µg, AMB0710-20 µg, AMB0710-50 µg, AMB0710-100 µg, AMB0710-250 µg, AMB0710-500 µg, AMB0710-1 mg, AMB0710-10, AMB0710-20, AMB0710-50, AMB0710-100, AMB0710-250, AMB0710-500, AMB0710-1

References & Citations

Jonathan D et al., (1996) Proc Natl Acad Sci USA 93:211-215.; ; Weiming Y et al., (2001) EMBO J 20 (3) :362-371.; ; Chen Z et al., (2005) Proc Natl Acad Sci USA 102 (29) :10200-10205.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Protein Marker

NCBI Accession Number

NP_418458.1

Uniprot Accession Number

P0AEX9

WB Description

The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MBP antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: MBP-tagged WLS protein (58.9kDa) ; ; Lane 2.: MBP-tagged IRF7 protein (59.3kDa) ;