Recombinant human Aldo-keto reductase 1B1/AKR1B1 protein
Aldose reductase (AKR1B1) is a member of the aldo-keto reductase (AKR) superfamily and catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. This protein is implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Recombinant Aldose reductase (AKR1B) protein was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE, Enzyme Activity
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Molecular Weight
35.8 kDa (316aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Ruiz FX., et al: (2009) Chem Biol Interact.178 (1-3) ; ; Donaqhue KC., et al: Diabet Med. 22 (10) :1315-20;
Other References
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
Scientific Category
Redox Proteins
NCBI Accession Number
NP_001619.1
Uniprot Accession Number
P15121
Species
Human
AA Sequence
Frequently Asked Questions
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