Recombinant human Aldehyde dehydrogenase 2/ALDH2 protein

ALDH2 (Aldehyde dehydrogenase 2 family) belongs to the aldehyde dehydrogenase family which catalyze the chemical transformation from acetaldehyde to acetic acid and is the second enzyme of the major oxidative pathway of alcohol metabolism. There are two major liver isoforms of this enzyme, cytosolic and mitochondrial, and they can be also distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Recombinant human ALDH2 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 250pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole Acetaldehyde by NAD per minute at pH 8.0 at 25°C.

Product Name Alternative

Aldehyde dehydrogenase 2 family member, Aldehyde dehydrogenase mitochondrial, ALDH class 2, ALDH-E2, ALDHI, ALDM

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 250pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole Acetaldehyde by NAD per minute at pH 8.0 at 25°C.

Molecular Weight

54.5 kDa (501aa) confirmed by MALDI-TOF

Additionnal Information

ALDH2, Aldehyde dehydrogenase mitochondrial, ALDH-E2, ALDHI, ALDM, Aldehyde dehydrogenase, Mitochondrial Acetaldehyde dehydrogenase 2, Aldehyde dehydrogenase 2 family, Aldehyde dehydrogenase 2 family (mitochondrial), ALDHE2, ALDH 2, ALDH class 2, ALDH E2, Liver mitochondrial ALDH, MGC1806, Mitochondrial aldehyde dehydrogenase 2, Nucleus encoded mitochondrial aldehyde dehydrogenase 2, ALD0905-10 µg, ALD0905-20 µg, ALD0905-50 µg, ALD0905-100 µg, ALD0905-250 µg, ALD0905-500 µg, ALD0905-1 mg, ALD0905-010, ALD0905-020, ALD0905-050, ALD0905-100, ALD0905-250, ALD0905-500, ALD0905-01M

References & Citations

Crabb DW., et al. (1989) . J Clin Invest. 83 (1) :314-6; ; Feng Liu., et al. (2002) . Plant Physiol. 130 (4) :1657-1674;

Other References

Hosoi T, et al. Flurbiprofen ameliorated obesity by attenuating leptin resistance induced by endoplasmic reticulum stress. (EMBO Mol Med. 2014) {https://pubmed.ncbi.nlm.nih.gov/24421337/}; ; Stiti N, et al. Nicotinamide Cofactors Suppress Active-Site Labeling of Aldehyde Dehydrogenases. (ACS Chem Biol. 2016) {https://pubmed.ncbi.nlm.nih.gov/26990764/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 1mM EDTA, 10% glycerol

Scientific Category

Redox Proteins

NCBI Accession Number

NP_000681.2

Uniprot Accession Number

P05091

Species

Human

AA Sequence

MSAAATQAVP APNQQPEVFC NQIFINNEWH DAVSRKTFPT VNPSTGEVIC QVAEGDKEDV DKAVKAARAA FQLGSPWRRM DASHRGRLLN RLADLIERDR TYLAALETLD NGKPYVISYL VDLDMVLKCL RYYAGWADKY HGKTIPIDGD FFSYTRHEPV GVCGQIIPWN FPLLMQAWKL GPALATGNVV VMKVAEQTPL TALYVANLIK EAGFPPGVVN IVPGFGPTAG AAIASHEDVD KVAFTGSTEI GRVIQVAAGS SNLKRVTLEL GGKSPNIIMS DADMDWAVEQ AHFALFFNQG QCCCAGSRTF VQEDIYDEFV ERSVARAKSR VVGNPFDSKT EQGPQVDETQ FKKILGYINT GKQEGAKLLC GGGIAADRGY FIQPTVFGDV QDGMTIAKEE IFGPVMQILK FKTIEEVVGR ANNSTYGLAA AVFTKDLDKA NYLSQALQAG TVWVNCYDVF GAQSPFGGYK MSGSGRELGE YGLQAYTEVK TVTVKVPQKN S