Human CD158b2/KIR2DL3 antibody

Reacts with extracellular domain of the KIR2DL3, a member of the Killer cell immunoglobulin-liKe receptor (KIR) family. The KIR family consists of transmembrane glycoproteins of the Ig superfamily expressed on human NK cells and a subset of human T cells which they are involved in recognition of either MHC class I molecules or unKnown ligand on target cells and inhibit cytotoxic activities.

Product Specifications

Product Name Alternative

Killer cell immunoglobulin like receptor, Two Ig domains and long cytoplasmic tail 3, Killer cell immunoglobulin-like receptor 2DL3, CD158 antigen-like family member B2, KIR-023GB, Killer inhibitory receptor cl 2-3, NKAT2a, NKAT2b, Natural killer-associated transcript 2, NKAT-2, p58 natural killer cell receptor clone CL-6, p58 NK receptor CL-6, p58.2 MHC class-I-specific NK receptor, CD158B2, KIRCL23, p58

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human KIR2DL3 (19-161aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

190IIC311

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

KIR2DL3, CD158b2, AKR3006-10 µg, AKR3006-20 µg, AKR3006-50 µg, AKR3006-100 µg, AKR3006-250 µg, AKR3006-500 µg, AKR3006-1 mg, AKR3006-10, AKR3006-20, AKR3006-50, AKR3006-100, AKR3006-250, AKR3006-500, AKR3006-1

References & Citations

Shin JS, et al., (1999) Hybridoma 18:521-527.; ; Chwae YJ, et al., (2002) J Immunology 169:3726-3735.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Immunology

NCBI Accession Number

NP_055326

Uniprot Accession Number

P43628

WB Description

The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KIR2DL3 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: KIR2DL1 recombinant protein; ; Lane 2.: KIR2DL3 recombinant protein; ; Lane 3.: KIR2DS4 recombinant protein

IF Description

ICC/IF analysis of KIR2DL3 in HeLa cells. The cell was stained with AKR3006 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .