Human KIR2DL1/KIR2DL3/KIR2DS4 antibody

The KIR family consists of transmembrane glycoproteins of the Ig superfamily expressed on human Nk cells and a subset of human T cells which they are involved in recognition of either MHC class I molecules or unknown ligand on target cells and inhibit cytotoxic activities.

Product Specifications

Product Name Alternative

Killer cell immunoglobulin like receptor two Ig domains and long cytoplasmic tail 1, Killer cell immunoglobulin-like receptor 2DL1, CD158 antigen-like family member A, Natural killer-associated transcript 1, NKAT-1, p58 natural killer cell receptor clones CL-42/47.11, p58 NK receptor CL-42/47.11, p58.1 MHC class-I-specific NK receptor, CD158a

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human kIR2DL1 (23-223 aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

2F9

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

KIR2DL3, P43628, NP_056952.2, KIR2DS4, P43632, NP_036446, Killer cell immunoglobulin-like receptor 2DL3, CD158 antigen-like family member B2, KIR-023GB, Killer inhibitory receptor cl 2-3, NKAT2a, NKAT2b, Natural killer-associated transcript 2, NKAT-2, p58 natural killer cell receptor clone CL-6, p58 NK receptor CL-6, p58.2 MHC class-I-specific NK receptor, CD158b2, KIRCL23, Killer cell immunoglobulin-like receptor 2DS4, CD158 antigen-like family member I, Natural killer-associated transcript 8, NKAT-8, P58 natural killer cell receptor clones CL-39/CL-17, p58 NK receptor CL-39/CL-17, CD158I, KKA3, KIR2DL1, CD158a, AKR0620-10 µg, AKR0620-20 µg, AKR0620-50 µg, AKR0620-100 µg, AKR0620-250 µg, AKR0620-500 µg, AKR0620-1 mg, AKR0620-010, AKR0620-020, AKR0620-050, AKR0620-100, AKR0620-250, AKR0620-500, AKR0620-01M

References & Citations

Shin JS, et al., 1999. Hybridoma 18:521-527.; ; Qing R. Fan, et al., 2001. Nature Immunology 2:452-460. ;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Immunology

NCBI Accession Number

NP_055033

Uniprot Accession Number

P43626

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KIR2DL1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HepG2 cell lysate; ; Lane 2.: Jurkat cell lysate The recombinant protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KIR2DL1 (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: KIR2DL1 recombinant protein; ; Lane 2.: KIR2DL3 recombinant protein; ; Lane 3.: KIR2DS4 recombinant protein; ; Lane 4.: KIR2DL4 recombinant protein {AKR0620-WB2.jpg}

FACS Description

Flow cytometry analysis of KIR2DL1 in Hep3B cell line, staining at 2-5ug for 1x10^6cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.

IF Description

ICC/IF analysis of KIR2DL1 in Hep3B cells. The cell was stained with AKR0620 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .