Human CD158i/KIR2DS4 antibody

The KIR family consists of transmembrane glycoproteins of the Ig superfamily expressed on human Nk cells and a subset of human T cells which they are involved in recognition of either MHC class I molecules or unknown ligand on target cells and inhibit cytotoxic activities. KIR2DS4 is an activating receptor of KIR family.

Product Specifications

Product Name Alternative

Killer cell immunoglobulin like receptor two Ig domains and short cytoplasmic tail 4, Killer cell immunoglobulin-like receptor 2DS4, CD158 antigen-like family member I, Natural killer-associated transcript 8, NKAT-8, P58 natural killer cell receptor clones CL-39/CL-17, p58 NK receptor CL-39/CL-17, KKA3, cl-39

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human kIR2DS4 (23-223aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

5F2

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

KIR2DS4, CD158i, AKR0504-10 µg, AKR0504-20 µg, AKR0504-50 µg, AKR0504-100 µg, AKR0504-250 µg, AKR0504-500 µg, AKR0504-1 mg, AKR0504-10, AKR0504-20, AKR0504-50, AKR0504-100, AKR0504-250, AKR0504-500, AKR0504-1

References & Citations

Steffens, U. et al. (1998) Tissue Antigens 51:398-413.; ; Kim, J. et al. (1997) J. Immunol. 159:3875-3882.; ; Wagtmann, N. R., et al. (1995) Immunity 2:439-449.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Immunology

NCBI Accession Number

NP_036446

Uniprot Accession Number

P43632

WB Description

The recombinant proteins (100ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KIR2DS4 antibody (1:1,000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human KIR2DL1 protein; ; Lane 2.: Recombinant human KIR2DL3 protein; ; Lane 3.: Recombinant human KIR2DL4 protein; ; Lane 4.: Recombinant human KIR2DS4 protein;