Human NKp46/NCR1 antibody

A natural cytotoxicity receptor (NCR), Nkp46, is a glycoprotein that has two extracellular Ig-like domains followed by a ~40 residue stalk resion, a type I transmembrane domain, and a short cytoplasmic tail. Nkp46 has been shown to represent a novel Nk cell-specific molecule involved in human Nk cell activation. The natural cytotoxicity receptors (NCRs) are a recently characterized family of Ig-like activation receptors that appear to be major triggering receptors in tumor cell recognition. Nkp46 has been implicated in Nk cell-mediated lysis of several autologous tumor cells and pathogen-infected cell lines.

Product Specifications

Product Name Alternative

NKp46 Extracellular Ig-like domain, NK-p46, NK cell-activating receptor, NCR1, NCR, Natural killer cell p46-related protein, Natural cytotoxicity triggering receptor 1 isoform a, Natural cytotoxicity triggering receptor 1, Lymphocyte antigen 94 homolog, Ly96, LY94, hNKp46, CD335 antigen

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human Nkp46 (22-255aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

N1D9

Conjugation

Unconjugated

Applications

ELISA, WB, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

NKp46 Extracellular Ig-like domain, NKp-46 Extracellular Ig-like domain, NKp46, NK p46, NK-p46, NK cell-activating receptor, NCR-1, NCR 1, NCR1, NCR, Natural killer cell p46-related protein, Natural cytotoxicity triggering receptor 1 isoform a, Natural cytotoxicity triggering receptor 1, Lymphocyte antigen 94 homolog, Ly96, LY94, hNKp46, CD335 antigen, AKR0408-10 µg, AKR0408-20 µg, AKR0408-50 µg, AKR0408-100 µg, AKR0408-250 µg, AKR0408-500 µg, AKR0408-1 mg, AKR0408-010, AKR0408-020, AKR0408-050, AKR0408-100, AKR0408-250, AKR0408-500, AKR0408-01M

References & Citations

Sivori, S. et al., (1997) J. Exp. Med. 186:1125-36.; ; Pessino, A. et al., (1998) J. Exp. Med. 188:953-60.

Other References

Ascierto ML, et al. Molecular signatures mostly associated with NK cells are predictive of relapse free survival in breast cancer patients. (J Transl Med. 2013) {https://pubmed.ncbi.nlm.nih.gov/23758773/}; ; Haberthur K, et al. NKG2D ligand expression in pediatric brain tumors. (Cancer Biol Ther. 2016) {https://pubmed.ncbi.nlm.nih.gov/27834580/}; ; Qu S, et al. Aneustat (OMN54) has aerobic glycolysis-inhibitory activity and also immunomodulatory activity as indicated by a first-generation PDX prostate cancer model. (Int J Cancer. 2018) {https://pubmed.ncbi.nlm.nih.gov/29441566/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Immunology

NCBI Accession Number

NP_004820

Uniprot Accession Number

O76036

WB Description

The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NKp46 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human NKp46 protein

FACS Description

Flow cytometry analysis of NKp46 in PBMC. The cell was stained with AKR0408 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black) .Flow cytometry analysis of NKp46 in HeLa cells. The cell was stained with AKR0408 at 2-5ug for 1x10^6 cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black) .{2004010009858312.jpg}