Human ISG15/UCRP antibody

ISG15, a 15 kDa protein encoded by an interferon stimulated gene, and its modification system play important roles in the innate immune response, regulation of interferon signaling, pregnancy, and several cancers. ISG15 is an ubiquitin-like protein containing two ubiquitin homology domains and becomes conjugated to a variety of proteins when cells are treated with type I interferon or lipopolysaccharide.

Product Specifications

Product Name Alternative

Interferon-stimulated gene 15 kDa ubiquitin-like modifier, GIP2, uCRP, IFI15, ISG15, Interferon-stimulated gene, 15 kDa ubiquitin-like modifier, Interferon-stimulated gene, 15 kDa ubiquitin-like modifier IFI 15, Interferon alpha inducible protein, Interferon induced 15 kDa protein, Interferon induced 17 kDa protein, Interferon induced 17 kDa/15 kDa protein, Interferon Stimulated Gene 15, Interferon stimulated protein 15 kDa, ISG 15, ISG15 ubiquitin like modifier, ubiquitin cross reactive protein precursor

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human ISG15 (1-157aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

300000

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

ISG15, UCRP, AIS0701-10 µg, AIS0701-20 µg, AIS0701-50 µg, AIS0701-100 µg, AIS0701-250 µg, AIS0701-500 µg, AIS0701-1 mg, AIS0701-10, AIS0701-20, AIS0701-50, AIS0701-100, AIS0701-250, AIS0701-500, AIS0701-1

References & Citations

Jonathan D et al., (1996) Proc Natl Acad Sci USA 93:211-215.; ; Weiming Y et al., (2001) EMBO J 20 (3) :362-371.; ; Chen Z et al., (2005) Proc Natl Acad Sci USA 102 (29) :10200-10205.

Other References

Zahoor MA, et al. HIV-1 Vpr Induces Interferon-Stimulated Genes in Human Monocyte-Derived Macrophages. (PLoS One. 2014) {https://pubmed.ncbi.nlm.nih.gov/25170834/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Ubiquitination

NCBI Accession Number

NP_005092

Uniprot Accession Number

P05161

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ISG15/UCRP antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1. : HeLa cell lysate; ; Lane 2. : MCF7 cell lysate

IF Description

ICC/IF analysis of ISG15 in HeLa cells. The cell was stained with AIS0701 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .