Human IRF5 antibody

Interferon regulatory factor 5 (IRF-5) is a member of IRF family, which plays a critical role in the regulation of genes induced by viral infection, immunostimulation and cell growth regulation. The expression of IRF-5 is limited to lymphoid organs, dendritic cells, and peripheral blood lymphocytes, but is not expressed in numerous leukemia and lymphoma cell lines. Three IRFs (IRF-3, IRF-5, IRF-7) function as direct transducers of virus-mediated signaling and play a critical role in the expression of type I interferon genes and some chemokines.

Product Specifications

Product Name Alternative

Interferon regulatory factor 5, irf5

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human IRF-5 (176-240aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

10T1

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, IHC, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

IRF5, AIR0611-10 µg, AIR0611-20 µg, AIR0611-50 µg, AIR0611-100 µg, AIR0611-250 µg, AIR0611-500 µg, AIR0611-1 mg, AIR0611-10, AIR0611-20, AIR0611-50, AIR0611-100, AIR0611-250, AIR0611-500, AIR0611-1

References & Citations

Mario B., et al, (2007) J. Virology 81 (9) :4473-4481.; ; Ning S., et al, (2005) J. Virology 79:11671-11676.

Other References

Barro M, Patton JT. Rotavirus NSP1 inhibits expression of type I interferon by antagonizing the function of interferon regulatory factors IRF3, IRF5, and IRF7. (J Virol. 2007) {https://pubmed.ncbi.nlm.nih.gov/17301153/}; ; Watanabe T, et al. Muramyl dipeptide activation of nucleotide-binding oligomerization domain 2 protects mice from experimental colitis. (J Clin Invest. 2008) {https://www.ncbi.nlm.nih.gov/pubmed/18188453}; ; Ye L, et al. Methamphetamine enhances Hepatitis C virus replication in human hepatocytes. (J Viral Hepat. 2008) {https://pubmed.ncbi.nlm.nih.gov/18307590/}; ; Pandey AK, et al. NOD2, RIP2 and IRF5 play a critical role in the type I interferon response to Mycobacterium tuberculosis. (PLoS Pathog. 2009) {https://www.ncbi.nlm.nih.gov/pubmed/19578435}; ; Polioudakis D, et al. A Myc-microRNA network promotes exit from quiescence by suppressing the interferon response and cell-cycle arrest genes. (Nucleic Acids Res. 2013) {https://pubmed.ncbi.nlm.nih.gov/23303785/}; ; Steinhagen F, et al. IRF-5 and NF-κB p50 co-regulate IFN-β and IL-6 expression in TLR9-stimulated human plasmacytoid dendritic cells. (Eur J Immunol. 2013) {https://pubmed.ncbi.nlm.nih.gov/23616277/}; ; He H, et al. Immobilized heavy chain-hyaluronic acid polarizes lipopolysaccharide-activated macrophages toward M2 phenotype. (J Biol Chem. 2013) {https://pubmed.ncbi.nlm.nih.gov/23878196/}; ; O Carroll C, et al. Identification of a unique hybrid macrophage-polarization state following recovery from lipopolysaccharide tolerance. (J Immunol. 2014) {https://pubmed.ncbi.nlm.nih.gov/24337373/}; ; Chionh YT, et al. Protease-activated receptor 1 suppresses Helicobacter pylori gastritis via the inhibition of macrophage cytokine secretion and interferon regulatory factor 5. (Mucosal Immunol. 2015) {https://pubmed.ncbi.nlm.nih.gov/24866378/}; ; Steinhagen F, et al. IRF5 and IRF8 modulate the CAL-1 human plasmacytoid dendritic cell line response following TLR9 ligation. (Eur J Immunol. 2016) {https://pubmed.ncbi.nlm.nih.gov/26613957/}; ; Li D, et al. Specific detection of interferon regulatory factor 5 (IRF5) : A case of antibody inequality. (Sci Rep. 2016) {https://pubmed.ncbi.nlm.nih.gov/27481535/}; ; Koelzer VH, et al. Digital analysis and epigenetic regulation of the signature of rejection in colorectal cancer. (Oncoimmunology. 2017) {https://pubmed.ncbi.nlm.nih.gov/28507795/}; ; Xiao Y, et al. An oligodeoxynucleotide with AAAG repeats significantly attenuates burn-induced systemic inflammatory responses via inhibiting interferon regulatory factor 5 pathway. (Mol Med. 2017) {https://pubmed.ncbi.nlm.nih.gov/28620671/}; ; Chen X, et al. MicroRNA-302a suppresses influenza A virus-stimulated interferon regulatory factor-5 expression and cytokine storm induction. (J Biol Chem. 2017) {https://pubmed.ncbi.nlm.nih.gov/29046356/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_116032.1

Uniprot Accession Number

Q13568

WB Description

The cell lysates of Ramos, THP-1, A20 and NIH3T3 were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IRF-5 antibody (1:1,000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

IHC description

Paraffin embedded sections of normal colon mucosa and colon adenocalcinoma tissue were incubated with anti-human IRF-5 antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) .

FACS Description

Flow cytometry analysis of IRF5 in THP-1 cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of IRF-5 in THP-1 cells. The cell was stained with AIR0611 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of IRF-5 in Raw264.7 cells. The cell was stained with AIR0611 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{AIR0611-IF2.jpg}