Human IL-33 antibody

Interleukin 33 (IL-33) is a 32kDa proinflammatory cytokine and intracellular nuclear factor with transcriptional regulatory properties. IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines and acts through the receptor IL1RL-1 (IL1 receptor-like-1), which is known also as ST2. Binding of IL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces expression of IL-4, IL-5, IL-13 and leads to severe pathological changes in mucosal organs and in vitro, it can be divided to N-terminal fragment of 12kDa and C-terminal fragment of 18kDa by cleavage of caspase-1.

Product Specifications

Product Name Alternative

Interleukin-33, Interleukin-1 family member 11, IL-1F11, Nuclear factor from high endothelial venules, NF-HEV, DVS27-related protein, DVS27, C9orf26

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human IL-33 (112-270aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

4000000000

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

IL33, AIL0824-10 µg, AIL0824-20 µg, AIL0824-50 µg, AIL0824-100 µg, AIL0824-250 µg, AIL0824-500 µg, AIL0824-1 mg, AIL0824-10, AIL0824-20, AIL0824-50, AIL0824-100, AIL0824-250, AIL0824-500, AIL0824-1

References & Citations

Brint Ek, et al., (2005) J Biol Chem. 20:277 (51) .; ; Schmitz, et al., (2005) immunity. 23:479.; ; Baekkevold ES, et al., (2003) Am.J.Pathol. 163 (1) :69-90.

Other References

Wang Y, et al. Exposure to cigarette smoke impacts myeloid-derived regulatory cell function and exacerbates airway hyper-responsiveness. (Lab Invest. 2014) {https://pubmed.ncbi.nlm.nih.gov/25365203/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Hormones & Cytokines, Angiogenesis, Cardiovascular

NCBI Accession Number

NP_254274

Uniprot Accession Number

O95760

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IL-33 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: Jurkat cell lysate;

FACS Description

Flow cytometry analysis of IL-33 in Jurkat cells. The cell was stained with AIL0824 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mousemonoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

IF Description

ICC/IF analysis of IL-33 in Jurkat cells. The cell was stained with AIL0824 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .