Human HSPH1 antibody

Hsp105 is a mammalian member of the HSP105/110 family, a diverged subgroup of the HSP70 family. HSP105 exists as two isoforms, alpha and beta. Hsp105 alpha associates with Hsp70/Hsc70 as complexes in vivo and regulates the chaperone activity of Hsp70/Hsc70 negatively in vitro and in vivo. Hsp105 alpha is constitutively expressed and also induced by various stress, whereas Hsp105beta, lacking 44 amino acids from Hsp105 alpha, is specifically expressed during mild heat shock. Although Hsp105 alpha is shown to localize in the cytoplasm of mammalian cells, cellular localization of Hsp105beta is not known.

Product Specifications

Product Name Alternative

Heat shock 105kDa/110kDa protein 1, HSPH1, Heat shock 105kDa/110kDa protein 1, HSP105alpha, HSP105-alpha, Heat shock 105kDa/110kDa protein 1 Antigen NY CO 25, DKFZp686M05240, Heat shock 105kD, Heat shock 105kD alpha, Heat shock 105kD beta, Heat shock 105kDa protein, Heat shock 105kDa protein 1, Heat shock 110kDa protein, Heat shock protein 105 kDa, HSP105A, HSP105B, HSP110, HSPH 1, KIAA0201, NY CO 25

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human Hsp105 alpha (1-858aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

J1G12

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

HSPH1, AHS0827-10 µg, AHS0827-20 µg, AHS0827-50 µg, AHS0827-100 µg, AHS0827-250 µg, AHS0827-500 µg, AHS0827-1 mg, AHS0827-10, AHS0827-20, AHS0827-50, AHS0827-100, AHS0827-250, AHS0827-500, AHS0827-1

References & Citations

Wang XY., et al. (2001) J Immunol. 166 (1) :490-7.; ; Yamagishi N., et al. (2006) Exp Cell Res. 312 (17) :3215-23. ; ; Saito Y., et al. (2007) Exp Cell Res. 313 (17) :3707-17.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_006635

Uniprot Accession Number

Q92598

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hsp105 a antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: HepG2 cell lysate ; ; Lane 3.: NIH-3T3 cell lysate ; ; Lane 4.: MCF7 cell lysate ; ; Lane 5.: U-87 MG cell lysate ; ; Lane 6.: A549 cell lysate ;

FACS Description

Flow cytometry analysis of Hsp105 alpha in Hep3B cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of Hsp105 alpha in Hep3B cells. The cell was stained with AHS0827 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .