Human HSP40/DNAJB1 antibody

Human Hsp40 (heat shock protein with molecular size of 40kDa) is one of the mammalian homologues of bacterial DnaJ heat shock protein and regulates cellular processes by aiding in the folding, transport and assembly. Hsp40 protein contains the J-domain which controls interaction with the ATPase domain of Dnak.

Product Specifications

Product Name Alternative

Heat shock 40 kDa protein 1, DNAJB1, DNAJ1, HDJ1, HSPF1, HSP40 (DNAJ), Heat shock 40 kDa protein 1, Heat shock protein 40, HSP40, DnaJ protein homolog 1, HDJ-1, DnaJ homolog subfamily B member 1, Heat shock 40 kDa protein 1, radial spoke 16 homolog B (Chlamydomonas), Hsp40, Sis1, RSPH16B

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human Hsp40 (1-340aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

K1C7

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

HSP40, DNAJB1, Heat shock 40 kDa protein 1, DNAJB1, DNAJ1, HDJ1, HSPF1, HSP40 (DNAJ), Heat shock 40 kDa protein 1, Heat shock protein 40, HSP40, DnaJ protein homolog 1, HDJ-1, DnaJ homolog subfamily B member 1, Heat shock 40 kDa protein 1, radial spoke 16 homolog B (Chlamydomonas), Hsp40, Sis1, RSPH16B, AHS0716-10 µg, AHS0716-20 µg, AHS0716-50 µg, AHS0716-100 µg, AHS0716-250 µg, AHS0716-500 µg, AHS0716-1 mg, AHS0716-10, AHS0716-20, AHS0716-50, AHS0716-100, AHS0716-250, AHS0716-500, AHS0716-1

References & Citations

Hata M et al., (1996) Genomics 38 (3) :446-449; ; Manishkumar et al., (2005) J Biol Chem 280 (48) :40041-40050; ; Christine M et al., (2007) Genetics 175 (4) :1649-1664

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 2,000. Recommended starting dilution is 1:1,000.

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NM_006145

Uniprot Accession Number

P25685

WB Description

The Cell lysates of Jurkat (50 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSP40 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSP40 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate ; ; Lane 2.: HSP40 Transfected 293T cell lysate {AHS0716-WB2.jpg}