Human HSP90 alpha antibody

Hsp90 is a human heat shock protein. In response to adverse change in their environment, cell from all organisms increase the expression of a class of proteins referred to as heat shock or stress protein. The Hsp90, a highly conserved stress-induced protein, is abundantly expressed in most tissues under non-stress conditions and is required for eukaryotic cell viability. Hsp90 is primarily a cytoplasmic protein and its function remains unknown. It exists in a dimeric form and has been observed to bind to several other cellular proteins such as retro-virus kinases, steroid receptor, heme-regulated protein kinase, actin and tubulin.

Product Specifications

Product Name Alternative

Heat shock protein HSP 90-alpha, HSP 86, Renal carcinoma antigen NY-REN-38, HSPC1, HSPCA, Heat shock protein 90-alpha Renal carcinoma antigen NY REN 38, D6S182, FLJ26984, FLJ31884, Heat shock 90kDa protein 1 alpha, heat shock protein 90kDa alpha (cytosolic), class A member 2, HSP84, HSP86, Hsp89, HSP89A, Hsp90, HSP90A, HSP90AA1, HSP90ALPHA, HSP90N, HSPCAL3, HSPCB, HSPN, LAP2, Lipopolysaccharide associated protein2, LPS associated protein 2, NY REN 38 antigen, hsp90, Heat shock protein HSP 90-alpha

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human Hsp90 (1-732aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

4F10

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, IHC, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

HSP90 a, AHS0704-10 µg, AHS0704-20 µg, AHS0704-50 µg, AHS0704-100 µg, AHS0704-250 µg, AHS0704-500 µg, AHS0704-1 mg, AHS0704-10, AHS0704-20, AHS0704-50, AHS0704-100, AHS0704-250, AHS0704-500, AHS0704-1

References & Citations

B-T, et al., (1984) Molecular & Cellular Biol 4: 2802-2810.; ; Pritchard, K. A, et al., (2001) J Biol Chem 276:17621-17624.; ; Miyamoto, A, et al., (2002) Nature 416:865-869.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_005339

Uniprot Accession Number

P07900

WB Description

The cell lysates (20 µg) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSP90 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate;

IHC description

Paraffin embedded sections of human urothelium were incubated with anti-human Hsp90 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

FACS Description

Flow cytometry analysis of HSP90 in HepG2 cells. The cell was stained with AHS0704 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

IF Description

ICC/IF analysis of HSP90 in HeLa cells. The cell was stained with AHS00704 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .