Human HSP70/HSPA1A antibody

Hsp70 is a human heat shock protein. Hsp70 is an important part of the cell's machinery for protein folding, and help to protect cells from stress. In most species, there are many proteins that belong to the hsp70 family. Some of these are only expressed under stress conditions, while some are present in cells under normal growth conditions and are not heat-inducible. Hsp70 can be found in different cellular compartments (nuclear, cytosolic, mitochondrial, endoplasmic reticulum, etc...), and the chaperone function of hsp70 is required for protection against stress-induced apoptosis.

Product Specifications

Product Name Alternative

Heat shock 70kDa protein 1A, Heat shock protein family A member 1A, HSP70, HSP70.1, HSP70-1/HSP70-2, HSPA1, HSPA1A, HSPA1B

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human Hsp70 (1-641aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

40000000

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, IHC

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Heat shock 70 kDa protein 1A, Heat shock 70kDa protein 1A, Heat shock protein family A member 1A, HSP 70, HSP70, HSP70.1, HSP70-1/HSP70-2, HSPA1, HSPA1A, HSPA1B, AHS0703-10 µg, AHS0703-20 µg, AHS0703-50 µg, AHS0703-100 µg, AHS0703-250 µg, AHS0703-500 µg, AHS0703-1 mg, AHS0703-010, AHS0703-020, AHS0703-050, AHS0703-100, AHS0703-250, AHS0703-500, AHS0703-01M

References & Citations

Lewis MJ, et al., (1985) EMBO J 4:3137-3143.; ; Gething M-J, et al., (1992) Nature 355:33-45.; ; Dick D.M, et al., (2000) Mol Cell Biol 20 (19) :7146-7159.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_005336

Uniprot Accession Number

P0DMV8

WB Description

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hsp70 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate; ; Lane 2.: HeLa cell lysate

IHC description

Paraffin embedded sections of human eccrine gland were incubated with anti-human Hsp70 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

IF Description

ICC/IF analysis of HSP70 in A549 cells line. The cell was stained with AHS0703 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .