Human HSP27 antibody

Hsp27 (also known as the Estrogen-Regulated 24k protein, and hsp 28) is a member of the mammalian small heat shock protein family. Hsp27 is expressed constitutively in many tissues and its expression is increased to high levels after various types of stress including elevated temperatures, toxic metals, drugs and oxidants. Also, Hsp27 is phosphorylated in vivo on three phosphorylation sites (Ser15, Ser78 and Ser82) by protein kinases including MAPkAP kinase 2 and the stress-activated protein kinase SAPk2 (p38) . Hsp27 forms a multimeric complex within cells, and its primary chaperone function, like other small Hsps, appears to be the stabilization of denatured or aggregated proteins in a refolding competent state.

Product Specifications

Product Name Alternative

Heat shock 27 kDa protein, Hspb1, Hsp25, Hsp27, Hsp27, HspB1, Heat shock 27 kDa protein, HSP 27, Growth-related 25 kDa protein, P25, HSP25, Heat shock protein beta-1, Heat shock 27 kDa protein 28 kDa heat shock protein, CMT2F, DKFZp586P1322, Estrogen regulated 24 kDa protein, at shock 25kDa protein 1, Heat shock 27kDa protein 1, Heat shock 28kDa protein 1, Heat Shock Protein 27, Heat shock protein beta 1, Heat Shock Protein27, HS.76067, Hsp 28, Hsp B1, Hsp28, HspB1, SRP27, Stress responsive protein 27.

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human Hsp27 (1-205aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

2A5

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, IHC, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

HSP27, AHS0702-10 µg, AHS0702-20 µg, AHS0702-50 µg, AHS0702-100 µg, AHS0702-250 µg, AHS0702-500 µg, AHS0702-1 mg, AHS0702-10, AHS0702-20, AHS0702-50, AHS0702-100, AHS0702-250, AHS0702-500, AHS0702-1

References & Citations

Ehrnsperger M, et al., (1997) EMBO J 16:221-229.; ; Steve J, et al., (2000) Mol Cell Biol 20 (20) :7602-7612.; ; Butt E, et al., (2001) J Biol Chem 276:7108-7113.; ; Michael, et al., (2002) Cell Stress Chsperones 7 (3) :281-296.

Other References

Kaiser F, et al. Association between circulating levels of heat-shock protein 27 and aggressive periodontitis. (Cell Stress Chaperones. 2018) {https://pubmed.ncbi.nlm.nih.gov/29766408/}; ; Eickholz P, et al. Effect of nonsurgical periodontal therapy on haematological parameters in grades B and C periodontitis: an exploratory analysis. (Clin Oral Investig. 2020) {https://pubmed.ncbi.nlm.nih.gov/32385656/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_001531

Uniprot Accession Number

P04792

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hsp27 (1:1,000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: K562 cell lysate; ; Lane 3.: MCF7 cell lysate The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hsp27 (1:1,000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: A431 cell lysate; ; Lane 2.: HepG2 cell lysate {AHS0702-WB2.jpg}

IHC description

Paraffin embedded sections of human colon cancer tissue were incubated with anti-human Hsp27 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

FACS Description

Flow cytometry analysis of Hsp27 in A549 cell line, staining at 2-5ug for 1x10^6cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Flow cytometry analysis of Hsp27 in HeLa cell line, staining at 2-5ug for 1x10^6cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. {AHS0702-FC2.jpg}

IF Description

ICC/IF analysis of Hsp27 in HeLa cells. The cell was stained with AHS0702 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .