Human Phosphoserine phosphatase/PSPH antibody

Human phosphoserine phosphatase (HPSP), specific for D- and L- phosphoserine, has been identified in all human tissues. HPSP is a Mg (2+) -dependent phosphoserine phosphatase. The three dimensional structure of HPSP reveals the structural and functional role of the divalent cation in the active site of phosphatases. In particular, the complex structures reveal that the open-closed environmental change of the active site, generated by local rearrangement of the alpha-helical bundle domain, is important for the substrate recognition and hydrolysis.

Product Specifications

Product Name Alternative

PSPH, PSPase, PSP, Phosphoserine phosphatase Human, Phosphoserine phosphatase deficiency, Phosphoserine phosphatase, O-phosphoserine phosphohydrolase, L-3-phosphoserine phosphatase, EC 3.1.3.3

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human PSP (1-225aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

3G12

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

PSPH, Phosphoserine phosphatase Human, PSPase, PSP, EC 3.1.3.3, O-phosphoserine phosphohydrolase, L-3-phosphoserine phosphatase, Phosphoserine phosphatase, L 3 phosphoserine phosphatase, O phosphoserine phosphohydrolase, Phosphoserine phosphatase deficiency, AHP0705-10 µg, AHP0705-20 µg, AHP0705-50 µg, AHP0705-100 µg, AHP0705-250 µg, AHP0705-500 µg, AHP0705-1 mg, AHP0705-010, AHP0705-020, AHP0705-050, AHP0705-100, AHP0705-250, AHP0705-500, AHP0705-01M

References & Citations

Moro-Furlani, et al., (1980) Ann Hum Genet 43 (4) :323-3233.; ; Kim HY, et al., (2002) J Biol Chem 277 (48) :46651-46658.; ; Peeraer Y, et al., (2004) Eur J Biochem 271 (16) :3421-3427.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_004568

Uniprot Accession Number

P78330

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PSP antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Jurkat cell lysate; ; Lane 2.: K562 cell lysate; ; Lane 3.: HepG2 cell lysate; ; Lane 4.: A549 cell lysate; ; Lane 5.: MCF7 cell lysate;

IF Description

ICC/IF analysis of PSP in A431 cells. The cell was stained with AHP0705 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .