Human Hexokinase 1/2 antibody

Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes (types 1 to 4 hexokinases) have been identified. These enzymes are found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase 2 is found in the skeletal muscle and includes hydrophobic N-terminal sequence capable of targeting the hexokinase to mitochondria.

Product Specifications

Product Name Alternative

BB404130, Brain form hexokinase, dea, EC 2.7.1.1, Glycolytic enzyme, HEXOKIN, Hexokinase, Hexokinase PI, Hexokinase type I, Hexokinase type II, Hexokinase-1, Hexokinase-2, Hexokinase-A, HK I, HK II, HK1, HK1 tb, HK1-s, HK1-ta, HK1-tc, HK2, HXK1, mHk1-s, Muscle form hexokinase, Tumor isozyme

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human Hexokinase 2 (1-917aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

1A7

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

BB404130, Brain form hexokinase, dea, EC 2.7.1.1, Glycolytic enzyme, HEXOKIN, Hexokinase, Hexokinase 1, Hexokinase 2, Hexokinase PI, Hexokinase type I, Hexokinase type II, Hexokinase1, Hexokinase-1, Hexokinase2, Hexokinase-2, Hexokinase-A, HK I, HK II, HK1, HK1 tb, Hk1-s, HK1-ta, HK1-tc, HK2, HKI, HKII, HXK 1, HXK 2, HXK1, HXK-1, HXK2, HXK-2, mHk1-s, Muscle form hexokinase, tumor isozyme, AHK0812-10 µg, AHK0812-20 µg, AHK0812-50 µg, AHK0812-100 µg, AHK0812-250 µg, AHK0812-500 µg, AHK0812-1 mg, AHK0812-010, AHK0812-020, AHK0812-050, AHK0812-100, AHK0812-250, AHK0812-500, AHK0812-01M

References & Citations

Furuta H, et al., (1996) Genomics 36 (1) :206-209.; ; Jon E, et al., (2003) J Exp Biology 206:2049-2057.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_000180

Uniprot Accession Number

P52789

WB Description

The recombinant proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase 1/2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Hexokinase 1 recombinant protein; ; Lane 2.: Hexokinase 2 recombinant protein; ; Lane 3.: Hexokinase 3 recombinant protein; ; Lane 4.: Hexokinase 4 recombinant protein The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase 1/2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: K562 cell lysate; ; Lane 2.: Jurkat cell lysate{AHK0812-WB2.jpg}

FACS Description

Flow cytometry analysis of Hexokinase 1/2 in Jurkat cells. The cell was stained with AHK0812 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

IF Description

ICC/IF analysis of Hexokinase 1/2 in Jurkat cells. The cell was stained with AHK0812 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .