Human Hexokinase antibody

Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase1 is found in the adipose tissue and liver and encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this hexokinase1 have been associated with hemolytic anemia due to hexokinase deficiency.

Product Specifications

Product Name Alternative

BB404130, Brain form hexokinase, dea, EC 2.7.1.1, Glycolytic enzyme, HEXOKIN, Hexokinase, Hexokinase PI, Hexokinase type I, Hexokinase-A, HK I, HK1 tb, Hk1-s, HK1-ta, HK1-tc, HXK1, mHk1-s, tumor isozyme, Hexokinase 1, Hexokinase 2, Hexokinase 3, Hexokinase 4, HK1, HK2, HK3, HK4

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human Hexokinase1 (1-917aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

4D7

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

BB404130, Brain form hexokinase, dea, EC 2.7.1.1, Glycolytic enzyme, HEXOKIN, Hexokinase, Hexokinase 1, Hexokinase 2, Hexokinase 3, Hexokinase PI, Hexokinase type I, Hexokinase type II, Hexokinase type III, Hexokinase1, Hexokinase2, Hexokinase3, Hexokinase-1, Hexokinase-2, Hexokinase-3, Hexokinase-A, HK I, HK II, HK III, HK1, HK1 tb, Hk1-s, HK1-ta, HK1-tc, HK2, HK3, HKI, HKII, HKIII, HXK1, HXK2, HXK3, HXK 1, HXK 2, HXK 3, HXK-1, HXK-2, HXK-3, mHk1-s, Muscle form hexokinase, Tumor isozyme, AHK0715-10 µg, AHK0715-20 µg, AHK0715-50 µg, AHK0715-100 µg, AHK0715-250 µg, AHK0715-500 µg, AHK0715-1 mg, AHK0715-010, AHK0715-020, AHK0715-050, AHK0715-100, AHK0715-250, AHK0715-500, AHK0715-01M

References & Citations

Ellison W R. et al., (1975) J Biol Chem 250:1864-1871.; ; Furuta H. et al., (1996) Genomics 36 (1) :206-9.; ; Jon E. et al., (2003) J.Exp Biology 206:2049-2057.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_000179

Uniprot Accession Number

P19367

WB Description

The recombinant proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Hexokinase 1 recombinant protein; ; Lane 2.: Hexokinase 2 recombinant protein; ; Lane 3.: Hexokinase 3 recombinant protein; ; Lane 4.: Hexokinase 4 recombinant protein; ; The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HepG2 cell lysate; ; Lane 2.: HeLa cell lysate; ; Lane 3.: Jurkat cell lysate; ; Lane 4.: K562 cell lysate; ; {AHK0715-WB2.jpg}The tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Mouse Brain Tissue lysate {AHK0715-WB3.jpg}

IF Description

ICC/IF analysis of Hexokinase in HeLa cells. The cell was stained with AHK0715 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .