Human GSK-3 beta antibody

Glycogen synthase kinase 3beta (GSk3beta) is a serine/threonine kinase involved in insulin, growth factor and Wnt signaling. Glycogen synthase kinase 3beta (GSk3beta) is a molecular regulator of cell fate in the central nervous system and a target of the phosphatidylinositol 3-kinase (PI3k) pathway. It is an enzyme with a diverse number of actions in intracellular signaling systems, regulating neuronal plasticity, gene expression and cell survival.

Product Specifications

Product Name Alternative

Glycogen synthase kinase 3 beta

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human GSK3 beta (341-420aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

1F7

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

GSK3 b, AGK0601-10 µg, AGK0601-20 µg, AGK0601-50 µg, AGK0601-100 µg, AGK0601-250 µg, AGK0601-500 µg, AGK0601-1 mg, AGK0601-10, AGK0601-20, AGK0601-50, AGK0601-100, AGK0601-250, AGK0601-500, AGK0601-1

References & Citations

Piyajit W et al., (2002) Proc Natl Acad Sci 99 (12) :7951-7955.; ; Rana D et al., (2003) EMBO J 22 (3) : 494-501.; ; Patrizia D S et al., (2006) Neurobiol Aging 27 (3) : 413-422.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_002084

Uniprot Accession Number

P49841

WB Description

The cell lysate of HeLa (30ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GSK3 beta (1:2000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

FACS Description

Flow cytometry analysis of GSK-3 beta in HeLa cells. The cell was stained with AGK0601 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

IF Description

ICC/IF analysis of GSK-3 beta in HeLa cells. The cell was stained with AGK0601 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .