Human Growth Hormone 1/GH1 antibody

Human growth hormone (hGH, somatotropin), a protein hormone from the anterior lobe of the pituitary gland, comprises a single polypeptide chain of 191 amino acid residues. The 20kDa variant form of human growth hormone (20kDa hGH) presents in extracts from pituitary glands and it differs from the major form of hGH (22kDa, 191 amino acid) by the deletion of amino acid residues 32-46. The growth hormone amino acid sequence varies considerably between species, and non-primate growth hormones have little activity in man. Main functions of hGH and 20kDa hGH are the stimulation of somatic and bone growth, as well as an increase in the size and mass of organs and tissues.

Product Specifications

Background

Neutralization:Measured by its ability to neutralize Growth hormone induced proliferation in Nb2-11 rat lymphoma cells. The neutralization ED50 is less than or equal to 2 µg/mL in the presence of 2.5 ng/mL recombinant protein human Growth hormone.{AGH0401-addpic.jpg}

Product Name Alternative

GH1, Growth Hormone, GH, GH-N, Pituitary growth hormone, Growth hormone 1, Somatotropin, GHN, hGH-N

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human growth hormone (27-217aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

G3H5

Conjugation

Unconjugated

Applications

ELISA, WB, Neutralization

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

GH, GH 1, GH1, GHN, GH-N, Growth Hormone, Growth hormone 1, hGH-N, Pituitary growth hormone, Somatotropin, AGH0401-10 µg, AGH0401-20 µg, AGH0401-50 µg, AGH0401-100 µg, AGH0401-250 µg, AGH0401-500 µg, AGH0401-1 mg, AGH0401-010, AGH0401-020, AGH0401-050, AGH0401-100, AGH0401-250, AGH0401-500, AGH0401-01M

References & Citations

Wickens, et al., (1978) J. Biol Chem. 253 (7) 2483-2495.; ; Chang, et al., (1978) Nature . 275,617-624.; ; Ullrich. et al., (1977) Science 196, 1313-1319.; ; Tsushima, et al., (2000) Endocr J. 47S17-2.; ; Horan, M. et al., (2003) Hum. Mutat. 21 (4) 408-423.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and Neutralization analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Growth factors

NCBI Accession Number

NP_000506

Uniprot Accession Number

P01241

WB Description

The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Growth hormone antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human hGH protein (21-217aa) ; ; Lane 2.: Recombinant human hGH protein (27-202aa)