Human FLIP antibody

FLIP, also known as CASP8 and FADD-like apoptosis regulator (CFLAR), contains two death effector domains (DEDs) and a caspase-like domain. FLIP may play a crucial role between cell survival and cell death pathway in mammalian cells and interacts with adapter protein FADD and caspase-8 and -10, and potently inhibits apoptosis induced by all known death receptors DR3 (death receptor 3), TRAIL-R (TNF-related apoptosis-inducing ligand receptor) and TNFR1 (tumor necrosis factor receptor 1) .

Product Specifications

Product Name Alternative

CASP8 and FADD-like apoptosis regulator isoform 1, CFLAR, CASH, CASP8AP1, CLARP, MRIT, Caspase homolog, Caspase-eight-related protein, Casper, Caspase-like apoptosis regulatory protein, Cellular FLICE-like inhibitory protein, FLAME-1, Inhibitor of FLICE, I-FLICE, MACH-related inducer of toxicity

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human FLIP (1-480aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT8B12

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

FLIP, AFL0926-10 µg, AFL0926-20 µg, AFL0926-50 µg, AFL0926-100 µg, AFL0926-250 µg, AFL0926-500 µg, AFL0926-1 mg, AFL0926-10, AFL0926-20, AFL0926-50, AFL0926-100, AFL0926-250, AFL0926-500, AFL0926-1

References & Citations

Yu JW, Shi Y., (2008) Oncogene. 27 (48) :6216-27.; ; Du C, et al., (2005) Kidney Int. 67 (4) :1397-409.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Apoptosis

NCBI Accession Number

NP_003870

Uniprot Accession Number

O15519

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FLIP antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Jurkat cell lysate; ; Lane 2.: HeLa cell lysate; ; Lane 3.: MCF7 cell lysate; ; Lane 4.: K562 cell lysate

IF Description

ICC/IF analysis of FLIP in HeLa cells. The cell was stained with AFL0926 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .