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Human FABP1/L-FABP antibody

FABP1 (Fatty acid binding protein1) encodes the fatty acid binding protein found in liver. FABP1 is composed of ten antiparallelbeta strands that form a barrel with a bigger binding pocket than the other FABPs allowing it to accommodate two fatty acid. This protein binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm; it may be involved in intracellular lipid transport and metabolism.

Product Specifications

Product Name Alternative

Fatty acid binding protein 1, Fatty acid-binding protein, liver, FABPL, L-FABP, FABP1, Fatty acid binding protein 1, fatty acid binding protein 1 liver

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human FABP1 (1-127aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

2G4

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, IHC, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

FABP1, L-FABP, AFA0908-10 µg, AFA0908-20 µg, AFA0908-50 µg, AFA0908-100 µg, AFA0908-250 µg, AFA0908-500 µg, AFA0908-1 mg, AFA0908-10, AFA0908-20, AFA0908-50, AFA0908-100, AFA0908-250, AFA0908-500, AFA0908-1

References & Citations

Atshaves BP, et al: (2004) Mol Cell Biochem. 259 (1-2), 115-29.; ; Nakamura T, et al: (2005) Diabestes Care. 28 (11), 2728-32.

Other References

Schlabritz-Loutsevitch N, et al. A first case of hepatocellular carcinoma in the baboon (Papio spp.) placenta. (J Med Primatol. 2019) {https://pubmed.ncbi.nlm.nih.gov/30246873/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_001434

Uniprot Accession Number

P07148

WB Description

The Recombinant protein (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FABP1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: FABP1 Recombinant protein ; ; Lane 2.: HepG2 cell lysate ; ; Lane 3.: Liver tissues lysate ;

IHC description

Paraffin embedded sections of human colon cancer tissue were incubated with anti-human FABP1 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

FACS Description

Flow cytometry analysis of FABP1 in Hep3B cell line, staining at 2-5ug for 1x10^6cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.

IF Description

ICC/IF analysis of FABP1 in Hep3B cells. The cell was stained with AFA0908 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .
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