Human ACOT11 antibody

ACOT11 (Acyl-CoA thioesterase11), also known as BFIT, is a protein with acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates. Expression of this protein in mouse has been associated with obesity and alternative splicing results in two transcript variants encoding different isoforms.

Product Specifications

Product Name Alternative

Thioesterase, adipose associated isoform BFIT2, acyl-CoA thioesterase 11, thioesterase, adipose associated, STARD14, BFIT, KIAA0707, BFIT1, THEM1, StAR-related lipid transfer (START) domain containing 14

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human ACOT11 (19-250aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

J4B2

Conjugation

Unconjugated

Applications

ELISA, WB, IHC

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

ACOT11, AFA0903-10 µg, AFA0903-20 µg, AFA0903-50 µg, AFA0903-100 µg, AFA0903-250 µg, AFA0903-500 µg, AFA0903-1 mg, AFA0903-10, AFA0903-20, AFA0903-50, AFA0903-100, AFA0903-250, AFA0903-500, AFA0903-1

References & Citations

S. H. Adams, et al: (2001) Biochem. J. 360, 135-142.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Angiogenesis

NCBI Accession Number

NP_671517

Uniprot Accession Number

Q8WXI4

WB Description

The cell lysates of LNCap, Hep3B (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human ACOT11 (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

IHC description

Paraffin embedded sections of human colon cancer tissue were incubated with anti-human ACOT11 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)