Human EphA2 antibody

The Eph family of receptors is the largest known subfamily of receptor tyrosine kinases (RTk) . Eph RTks and their ligands, the ephrins, are frequently overexpressed in a variety of cancers and tumor cell lines. One Eph family member, EphA2, is also overexpressed in a wide variety of cancers, including breast, small-cell lung and gastrointestinal cancers, melanomas, and neuroblastomas. The ephrin-Eph interactions are important in development, especially in cell-cell interactions involved in nervous system patterning and possibly in cancer.

Product Specifications

Product Name Alternative

EPH receptor A2, Ephrin type-A receptor 2, EPHA2, Epithelial cell kinase, Tyrosine-protein kinase receptor ECK, ARCC2, CTPA, CTPP1, CTRCT6

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human EphA2 (559-976aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

3D7

Conjugation

Unconjugated

Applications

ELISA, WB, IHC

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

EphA2, Ephrin receptor EphA2, epoxide hydrolase 1 microsomal (xenobiotic), AEA0909-10 µg, AEA0909-20 µg, AEA0909-50 µg, AEA0909-100 µg, AEA0909-250 µg, AEA0909-500 µg, AEA0909-1 mg, AEA0909-10, AEA0909-20, AEA0909-50, AEA0909-100, AEA0909-250, AEA0909-500, AEA0909-1

References & Citations

Ireton RC, Chen J: (2005) Curr Cancer Drug Tarqets. 5 (3), 149-57; ; Nakamoto M, Berqemann AD: (2002) Microsc Res Tech. 59 (1), 58-67

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_004422

Uniprot Accession Number

P29317

WB Description

The cell lysates (10 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EphA2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate ; ; Lane 2.: EphA2 extracellular domain transfected 293T cell lysate ; ; Lane 3.: EphA2 cytoplasmic domain transfected 293T cell lysate

IHC description

Paraffin embedded sections of human colon cancer tissue were incubated with anti-human EphA2 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) Paraffin embedded sections of human gastric cancer tissue were incubated with anti-human EphA2 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) {AEA0909-IHC1b.jpg}