Recombinant human AK4 (C22S) protein
Adenylate kinase (AK; adenosine triphosphate-adenosine monophosphate [ATP-AMP] phospho-transferase, EC 2. 7. 4. 3) is a ubiquitous monomeric enzyme involved energy metabolism of prokaryotic and eukaryotic cells. Five isozymes of adenylate kinase have been identified in vertebrates. AK1 is present in the cytosol of skeletal muscle, brain, and erythrocyte, while AK2 is localized in the intermembrane space of mitochondria of liver, kidney, spleen and heart. AK3, called GTP:AMP phosphotransferase, exists in the mitochondrial matrix of liver and heart. These isozymes contribute to homeostasis of the adenine nucleotide composition in the cell. Recombinant human AK3, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
AK3, AKL3L, Adenylate kinase 3 alpha like 1, Adenylate kinase 3, AK3L1, AK6, AKL3L1, FIX, RP11 6J24.4.
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
29.3 kDa (259aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Nobumoto M., et al. (1998) J. Biol. Chem. 123 (1) :128-35; ; Noma T., et al. (2001) J. Biol. Chem. 358 (Pt 1) :225-32;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 2mM DTT, 30% glycerol, 0.1M NaCl
Scientific Category
Signal Transduction
NCBI Accession Number
NP_982289
Uniprot Accession Number
P27144
Species
Human
AA Sequence
Frequently Asked Questions
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