Human DJ-1/PARK7 antibody

Park7/DJ-1, which is a small 189 amino acid protein, is a ubiquitously expressed protein involved in various cellular processes including spermatogenesis and fertilization, cancer, RNA-binding, androgen-receptor signaling and oxidative stress. Mutations in the Park7/DJ-1 are the cause of autosomal recessive early-onset Parkinson's disease 7 (Park7) .

Product Specifications

Product Name Alternative

Parkinson disease protein 7, Parkinsonism associated deglycase, parkinson protein 7, Parkinson disease autosomal recessive early onset 7, Maillard deglycase, Oncogene DJ1, Protein DJ-1, DJ-1, DJ1, GATD2, Protein/nucleic acid deglycase DJ-1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human Park7/DJ-1 (1-189 aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

1B11

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

DJ1, DJ-1, DJ 1, DJ1 protein, GATD2, Maillard deglycase, Oncogene DJ1, PARK7, Parkinson disease autosomal recessive early onset 7, Parkinson disease protein 7, Parkinson disease protein 7 CAP1, parkinson protein 7, Parkinsonism associated deglycase, Protein DJ-1, Protein/nucleic acid deglycase DJ-1, SP22, PARK 7, ADJ0618-10 µg, ADJ0618-20 µg, ADJ0618-50 µg, ADJ0618-100 µg, ADJ0618-250 µg, ADJ0618-500 µg, ADJ0618-1 mg, ADJ0618-010, ADJ0618-020, ADJ0618-050, ADJ0618-100, ADJ0618-250, ADJ0618-500, ADJ0618-01M

References & Citations

Meulener MC. et al (2005) J. Neurochem. 93, 1524-1532.; ; Bonifati V. et al (2003) Science. 299, 256-259.; ; Zhou W. et al (2005) J. Biol. Chem. 280, 43150-43158.; ; Junn E. et al (2005) PNAS. 102, 9691-9696.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Neuroscience

NCBI Accession Number

NP_009193

Uniprot Accession Number

Q99497

WB Description

The cell lysate of Jurkat was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Park7/DJ-1 antibody (1:1000) . Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.