Recombinant mouse Adiponecticrp30 protein

Adiponectin is an adipocyte specific secreted protein that circulates in the plasma. It is induced during adipocyte differentiation and its secretion is stimulated by insulin. Mouse adiponectin shares about 83% amino acid identity with that human. Adiponectin plays a role in various physiological processes such as energy homeostasis and obesity. Adiponectin is reduced in obese humans, and decreased level is associated with insulin resistance and hyperinsulinemia. Recombinant mouse adiponectin, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

ADIPOQ, Acdc, Acrp30, Apm1, 30 kDa adipocyte complement-related protein, Adipocyte complement-related 30 kDa protein, Adipocyte C1q and collagen domain-containing protein, Adipocyte-specific protein

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

27.2 kDa (251aa) confirmed by MALDI-TOF

Additionnal Information

Adiponectin, Acrp30, adipocyte complement-related protein, Acdc, GBP28, Acrp30, mAdiponectin, 18- 247aa, Mouse, His-tagged, Adiponectin, C1Q and collagen domain containing [Mus musculus], ADI0702-10 µg, ADI0702-20 µg, ADI0702-50 µg, ADI0702-100 µg, ADI0702-250 µg, ADI0702-500 µg, ADI0702-1 mg, ADI0702-10, ADI0702-20, ADI0702-50, ADI0702-100, ADI0702-250, ADI0702-500, ADI0702-1

References & Citations

Yokota. T. et al. (2000) Blood. 96:1723; ; Yamauchi T. et al (2002) Nature Medicine.8 (11) :1288-1295; ; Lu M. et al (2007) Mol Endocrinol. 59 (2) :207-16;

Other References

Jeon BT, et al. Adiponectin protects hippocampal neurons against kainic acid-induced excitotoxicity. (Brain Res Rev. 2009) {https://pubmed.ncbi.nlm.nih.gov/19460404/}; ; Suzuki Y, et al. Chemerin is a novel regulator of lactogenesis in bovine mammary epithelial cells. (Biochem Biophys Res Commun. 2015) {https://pubmed.ncbi.nlm.nih.gov/26342800/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_033735

Uniprot Accession Number

Q60994

Species

Mouse

AA Sequence

EDDVTTTEE LAPALVPPPK GTCAGWMAGI PGHPGHNGTP GRDGRDGTPG EKGEKGDAGL LGPKGETGDV GMTGAEGPRG FPGTPGRKGE PGEAAYVYRS AFSVGLETRV TVPNVPIRFT KIFYNQQNHY DGSTGKFYCN IPGLYYFSYH ITVYMKDVKV SLFKKDKAVL FTYDQYQEKN VDQASGSVLL HLEVGDQVWL QVYGDGDHNG LYADNVNDST FTGFLLYHDT N