Human Cathepsin L antibody

Cathepsin L, a lysosomal endopeptidase expressed in most eukaryotic cells, is a member of the papain-like family of cysteine proteinases. Cathepsin L can be induced by many different signaling events, including growth factor, secondary messenger and tumor promoters. Cathepsin L is translated as preprocathepsin L, transferred through the Golgi apparatus as procathepsin L and then stored in lysosomes as mature cathepsin L.

Product Specifications

Product Name Alternative

Procathepsin L, Cathepsin L1, Major excreted protein, MEP, CTSL, CTSL1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human CTSL (18-333aa) purified from E. coli.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT18F6

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Cathepsin L, ACT0905-10 µg, ACT0905-20 µg, ACT0905-50 µg, ACT0905-100 µg, ACT0905-250 µg, ACT0905-500 µg, ACT0905-1 mg, ACT0905-10, ACT0905-20, ACT0905-50, ACT0905-100, ACT0905-250, ACT0905-500, ACT0905-1

References & Citations

Zheng X., et al (2009) Am J Physiol Cell Physiol. 296 (1) : 65-74.; ; Reinheckel T., et al (2005) J Cell Sci. 118: 3387-95.; ; Ishidoh K., Kominami E. (2002) Biol chem. 383 (12) : 1827-31.; ; Roth W., et al (2000) FASEB J. 14 (13) : 2075-86.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_001244901

Uniprot Accession Number

P07711

WB Description

The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTSL antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: A549 cell lysate The recombinant proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTSL antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: CTSB recombinant protein ; ; Lane 2.: CTSD recombinant protein ; ; Lane 3.: CTSE recombinant protein ; ; Lane 4.: CTSF recombinant protein ; ; Lane 5.: CTSH recombinant protein ; ; Lane 6.: CTSK recombinant protein ; ; Lane 7.: CTSL recombinant protein ; ; Lane 8.: CTSS recombinant protein ; ; Lane 9.: CTSW recombinant protein ; ; Lane 10.: CTSZ recombinant protein {ACT0905-WB2.jpg}

FACS Description

Flow cytometry analysis of CTSL in A549 cells. The cell was stained with ACT0905 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

IF Description

ICC/IF analysis of CTSL in A549 cells. The cell was stained with ACT0905 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .