Human Cystatin B antibody

CSTB, also known as Cystatin B is an anti-protease implicated in myoclonus epilepsy, a degenerative disease of the central nervous system. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. This protein is able to from a dimer stabilized by noncovalent forces and is thought to play a role in protecting against the proteases leaking from lysosomes in cells. CSTB is located in the lysosomes and the cytoplasm, but also in the nucleus.

Product Specifications

Product Name Alternative

CystatinB, Cystatin B, CSTB, Stefin B, PME, CST6, CystatinB CHROW21, CPI B, CYTB, EPM1, Liver thiol proteinase inhibitor, STFB

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human CSTB (1-98aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

2F1

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Cystatin B, ACS0820-10 µg, ACS0820-20 µg, ACS0820-50 µg, ACS0820-100 µg, ACS0820-250 µg, ACS0820-500 µg, ACS0820-1 mg, ACS0820-10, ACS0820-20, ACS0820-50, ACS0820-100, ACS0820-250, ACS0820-500, ACS0820-1

References & Citations

Turk V, et al., (1991) FEBS Lett. 285 (2) :213-9.; ; Jarvinen M, et al., (1988) Acta Histochem. 82 (1) :5-18.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Neuroscience

NCBI Accession Number

NP_000091

Uniprot Accession Number

P04080

WB Description

The cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CSTB antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: A549 cell lysate (35ug) ; ; Lane 2.: U87MG cell lysate (40ug)

IF Description

ICC/IF analysis of CSTB in A549 cells. The cell was stained with ACS0820 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .