Human C-Reactive Protein/CRP antibody

C-reactive protein (CRP) is an acute-phase protein with a well-known association with infection and other inflammatory conditions. Mild elevations in C-reactive protein concentration predict myocardial infarction, stroke, and vascular death in a variety of clinical settings. Despite the lack of specific evidence that C-reactive protein levels are independently associated with cardiovascular risk in patients with hypertension, the prognostic value of C-reactive protein has proven to be complementary to that of blood pressure values.

Product Specifications

Product Name Alternative

C-reactive protein (pentraxin-related), PTX1, MGC88244, MGC149895, C-reactive protein pentraxin-related, pentraxin 1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human CRP (19-224aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

5A9

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

C-Reactive Protein, CRP, ACR0631-10 µg, ACR0631-20 µg, ACR0631-50 µg, ACR0631-100 µg, ACR0631-250 µg, ACR0631-500 µg, ACR0631-1 mg, ACR0631-10, ACR0631-20, ACR0631-50, ACR0631-100, ACR0631-250, ACR0631-500, ACR0631-1

References & Citations

Alexander J. S et al., (2000) Infect Immun 68 (10) :5652-5656.; ; Schillaci G. et al., (2006) Nutr Metab Cardiovasc Dis. 16 (7) :500-8.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Apoptosis

NCBI Accession Number

NP_000558

Uniprot Accession Number

P02741

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRP antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: NIH-3T3 cell lysate; ; Lane 2.: HepG2 cell lysate; ; Lane 3.: Jurkat cell lysate; ; Lane 4.: HeLa cell lysate; ; Lane 5.: SH-SY5Y cell lysate

FACS Description

Flow cytometry analysis of CRP in HepG2 cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of CRP in HepG2 cells. The cell was stained with ACR0631 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .