Human Cofilin 1/CFL1 antibody

Cofilin is a family of actin-binding proteins which disassembles actin filaments. It is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.

Product Specifications

Product Name Alternative

18 kDa phosphoprotein (p18), CFL, CFL1, Cofilin 1, Cofilin 1 non muscle, Cofilin non muscle isoform, P18

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human Cofilin 1 (1-166aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT1C1

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

18 kDa phosphoprotein, 18 kDa phosphoprotein (p18), 18kDa phosphoprotein (p18), CFL, CFL-1, CFL 1, CFL1, Cofilin 1, Cofilin1, Cofilin-1, Cofilin 1 non muscle, Cofilin non muscle isoform, P18, ACO0927-10 µg, ACO0927-20 µg, ACO0927-50 µg, ACO0927-100 µg, ACO0927-250 µg, ACO0927-500 µg, ACO0927-1 mg, ACO0927-010, ACO0927-020, ACO0927-050, ACO0927-100, ACO0927-250, ACO0927-500, ACO0927-01M

References & Citations

Mannherz H.G., et al. (2007) J Mol Biol 366 (3) :745-755.; ; Olsen J.V., et al. (2006) Cell 127 (3) :635-648.; ; Yang N., et al. (1998) Nature 393 (6687) :809-812.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cytoskeleton

NCBI Accession Number

NP_005498

Uniprot Accession Number

P23528

WB Description

The lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cofilin 1 (1:1000) antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: K562 cell lysate; ; Lane 3.: MCF7 cell lysate; ; Lane 4.: LnCap cell lysate; ; Lane 5.: PC3 cell lysate

IF Description

ICC/IF analysis of Cofilin1 in HeLa cells. The cell was stained with ACO0927 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .