Human Clusterin/CLU antibody

Clusterin has been implicated in numerous processes including active cell death, immune regulation, cell adhesion and morphological transformation. Clusterin (previousiy named SP-40) is a glycoprotein, 80kDa comprising two distinct 40kDa polypeptide chains. It shows marked similarity, both physicochemically and in immunohistological distribution, to the single chain, 80kDa, Sprotein, an additional component of the SC5b-9 complex. Clusterin is a secreted glycoprotein of which the exact function remains a matter for speculation.

Product Specifications

Product Name Alternative

Clusterin, ApoJ, clusterin (complement lysis inhibitor SP-40,40, sulfated glycoprotein 2 testosterone-repressed prostate message 2 apolipoprotein J), SGP-2, SP-40, TRPM-2, KUB1, complement lysis inhibitor, ulfated glycoprotein 2, testosterone-repressed prostate message 2, apolipoprotein J

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human Clusterin (1-333aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

1A11

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, IHC, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Clusterin, ACL0711-10 µg, ACL0711-20 µg, ACL0711-50 µg, ACL0711-100 µg, ACL0711-250 µg, ACL0711-500 µg, ACL0711-1 mg, ACL0711-10, ACL0711-20, ACL0711-50, ACL0711-100, ACL0711-250, ACL0711-500, ACL0711-1

References & Citations

Maximino R et al., (2000) Am J Pathol 157 (2) :393-399.; ; Ingrid C.G et al., (1998) Biochem J 331:231-237.; ; Maximino R et al., (1990) J Clin Invest 85:1477-1486.; ; Fabien L et al., (2006) Biochem J 395 (1) :223-231.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Immunology

NCBI Accession Number

NP_001822

Uniprot Accession Number

P10909

WB Description

The cell lysate of U87MG (20 µg) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Clusterin (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

IHC description

Paraffin embedded sections of human renal cell carcinoma tissue were incubated with anti-human Clusterin (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

FACS Description

Flow cytometry analysis of Clusterin in U87MG cell line, staining at 2-5ug for 1x10^6cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.

IF Description

ICC/IF analysis of Clusterin in U87MG cells. The cell was stained with ACL0711 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .