Human CIB1 antibody

CIB1 (Calcium and integrin binding 1) is regulatory protein with 50% homology to calmodulin and calcineurin B, that encodes a member of the calcium-binding protein family. The specific function of this protein has not yet been determined; however this protein is known to interact with DNA-dependent protein kinase and may play a role in kinase-phosphatase regulation of DNA end joining. Also CIB1 is widely expressed and binds to a number of effectors, such as integrin alphaIIb, PAk1, and polo-like kinases in different tissues.

Product Specifications

Product Name Alternative

Calcium and integrin binding 1, CIB, KIP (Kinase interacting protein), SIP2-28 (SNK interacting protein2-28), CALMYRIN, CIB1, Calcium and integrin binding 1 Calcium and integrin binding 1 (calmyrin), Calcium and integrin binding protein 1, CIB, DNA PKcs interacting protein, Human Snk interacting protein 2 to 28 mRNA complete cds, Kinase interacting protein, PRKDCIP, SIP2 28, SNK interacting protein 2 to 28

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human CIB1 (1-191aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

1D1

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

CIB1, ACI0807-10 µg, ACI0807-20 µg, ACI0807-50 µg, ACI0807-100 µg, ACI0807-250 µg, ACI0807-500 µg, ACI0807-1 mg, ACI0807-10, ACI0807-20, ACI0807-50, ACI0807-100, ACI0807-250, ACI0807-500, ACI0807-1

References & Citations

Yuan W, et al., (2006) Mol Cell Biol 26 (22) : 8507-14.; ; Yuan W, et al., (2006) J Cell Biol 16; ; 172 (2) :169-75.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_006375

Uniprot Accession Number

Q99828

WB Description

The cell lysate of HT29 (40ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CIB1 (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysates (10 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CIB1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: CIB1 Transfected 293T cell lysate {ACI0807-WB2.jpg}

IF Description

ICC/IF analysis of CIB1 in HeLa cells. The cell was stained with ACI0807 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .