Human Integrin beta 1/CD29 antibody

CD29, also known as Integrin beta 1, is the beta subunit found in the integrin families, forming a heterodimer integrin receptor through non-covalent bonding with various integrin alpha subunits. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. Interaction between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression.

Product Specifications

Product Name Alternative

Integrin beta 1, Integrin beta 1, CD29, FNRB, MDF2, VLAB, GPIIA, MSK12, Fibronectin receptor subunit beta, Glycoprotein Iia, GPIIA, VLA-4 subunit bet

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human CD29 (34-141aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

K2D5

Conjugation

Unconjugated

Applications

ELISA, ICC/IF, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

VLA-BETA, VLAB, VLA-4 subunit beta, NRK2, Nicotinamide riboside kinase 2, MSK12, MIBP, MDF2, ITGB1, Interleukin-6, Interleukin6, Interleukin 6, Interferon beta 2, Integrin beta-1 isoform 1A, Integrin beta 1, Integrin b1, Il-6, IL6, IFNB2, IFNB 2, IFN B2, IFN B 2, I L6, HSF, HGF, GPIIA, Glycoprotein Iia, FNRB, Fibronectin receptor subunit beta, CD29, BSF2, BSF 2, ACD0823-10 µg, ACD0823-20 µg, ACD0823-50 µg, ACD0823-100 µg, ACD0823-250 µg, ACD0823-500 µg, ACD0823-1 mg, ACD0823-010, ACD0823-020, ACD0823-050, ACD0823-100, ACD0823-250, ACD0823-500, ACD0823-01M

References & Citations

He L, et al., (2003) Blood 102 (10) :3652-3657.; ; Arrequi C, et al., (2000) J cell Biol 149 (6) :1263-1274.; ; Martin-padura I, et al., (1994) J Biol Chem 269:6124-6132.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Immunology

NCBI Accession Number

NP_002202

Uniprot Accession Number

P05556

FACS Description

Flow cytometry analysis of CD29 in Hep3B cell line, staining at 2-5ug for 1x10^6cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.

IF Description

ICC/IF analysis of CD29 in HepG2 cells. The cell was stained with ACD0823 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .