Human 5′-Nucleotidase/CD73 antibody

CD73 (Ecto-5-nucleotidase, 5-NT) is a membrane-bound glycoprotein that functions to hydrolyze extracellular nucleoside monophosphates into bioactive nucleoside intermediates. This enables the uptake of adenosine, inosine, and guanosine into the cell and their subsequent reconversion into ATP and GTP in the purine salvage pathway. The expression of CD73 on neuronal cells has been described during developmental processes and, on lymphocytes, CD73 serves as a maturation marker, being absent from the surface of both immature B and T cells.

Product Specifications

Product Name Alternative

CD73, 5-NT, 5'-nucleotidase, 5'-nucleotidase ecto, AI447961, E5NT, ecto, eN, eNT, NT, NT5, Nt5e, NTE, 2210401F01Rik

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human CD73 (27-252aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT89E9

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Purine 5-Prime-Nucleotidase, NTE, NT5E, NT5, NT, eNT, eN, ecto-5'-nucleotidase, ecto, CD73, CALJA, AI447961, 5-nucleotidase ecto, 5'-nucleotidase ecto, 5-nucleotidase, 5'-nucleotidase, 5-NT, 5'-NT, 5' nucleotidase ecto, 5 nucleotidase ecto, 5' nucleotidase, 5 nucleotidase, 5' NT, 2210401F01Rik, ACD0806-10 µg, ACD0806-20 µg, ACD0806-50 µg, ACD0806-100 µg, ACD0806-250 µg, ACD0806-500 µg, ACD0806-1 mg, ACD0806-010, ACD0806-020, ACD0806-050, ACD0806-100, ACD0806-250, ACD0806-500, ACD0806-01M

References & Citations

Sern P.C, et al., (2006) Purinergic Signal 2 (2) :351-360.; ; Linda F.T, et al., (2004) J Exp Med 200 (11) :1395-1405.; ; Laura A, et al., (1997) J Cell Biol 136 (2) :421-431.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Immunology

NCBI Accession Number

NP_002517

Uniprot Accession Number

P21589

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human 5'-Nucleotidase/CD73 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HepG2 cell lysate; ; Lane 2.: WiDr cell lysate; ; Lane 3.: A431 cell lysate;

FACS Description

Flow cytometry analysis of 5'-Nucleotidase/CD73 in A431 cells. The cell was stained with ACD0806 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mousemonoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

IF Description

ICC/IF analysis of 5'-Nucleotidase/CD73 in HeLa cells. The cell was stained with ACD0806 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .